Graham T Dempsey
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Explore the profile of Graham T Dempsey including associated specialties, affiliations and a list of published articles.
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25
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1826
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Recent Articles
21.
Dempsey G, Vaughan J, Chen K, Bates M, Zhuang X
Nat Methods
. 2011 Nov;
8(12):1027-36.
PMID: 22056676
One approach to super-resolution fluorescence imaging uses sequential activation and localization of individual fluorophores to achieve high spatial resolution. Essential to this technique is the choice of fluorescent probes; the...
22.
Dempsey G, Bates M, Kowtoniuk W, Liu D, Tsien R, Zhuang X
J Am Chem Soc
. 2009 Dec;
131(51):18192-3.
PMID: 19961226
Cyanine dyes have been shown to undergo reversible photoswitching, where the fluorophore can be switched between a fluorescent state and a dark state upon illumination at different wavelengths. The photochemical...
23.
Fast in vitro translation system immobilized on a surface via specific biotinylation of the ribosome
Stapulionis R, Wang Y, Dempsey G, Khudaravalli R, Nielsen K, Cooperman B, et al.
Biol Chem
. 2008 Aug;
389(9):1239-49.
PMID: 18713011
The ribosome is the macromolecular machine responsible for translating the genetic code into polypeptide chains. Despite impressive structural and kinetic studies of the translation process, a number of challenges remain...
24.
Wang Y, Qin H, Kudaravalli R, Kirillov S, Dempsey G, Pan D, et al.
Biochemistry
. 2007 Aug;
46(38):10767-75.
PMID: 17727272
EF-G catalyzes translocation of mRNA and tRNAs within the ribosome during protein synthesis. Detection of structural states in the reaction sequence that are not highly populated can be facilitated by...
25.
Bates M, Huang B, Dempsey G, Zhuang X
Science
. 2007 Aug;
317(5845):1749-53.
PMID: 17702910
Recent advances in far-field optical nanoscopy have enabled fluorescence imaging with a spatial resolution of 20 to 50 nanometers. Multicolor super-resolution imaging, however, remains a challenging task. Here, we introduce...