Enhlink Infers Distal and Context-specific Enhancer-promoter Linkages

Overview

Journal Genome Biol

Specialties Biology
Genetics

Date 2024 Sep 2

PMID 39223609

Authors

Olivier B Poirion

Wulin Zuo

Catrina Spruce

Candice N Baker

Sandra L Daigle

Ashley Olson

Daniel A Skelly

Elissa J Chesler

Christopher L Baker

Brian S White

Affiliations

Soon will be listed here.

Abstract

Enhlink is a computational tool for scATAC-seq data analysis, facilitating precise interrogation of enhancer function at the single-cell level. It employs an ensemble approach incorporating technical and biological covariates to infer condition-specific regulatory DNA linkages. Enhlink can integrate multi-omic data for enhanced specificity, when available. Evaluation with simulated and real data, including multi-omic datasets from the mouse striatum and novel promoter capture Hi-C data, demonstrate that Enhlink outperfoms alternative methods. Coupled with eQTL analysis, it identified a putative super-enhancer in striatal neurons. Overall, Enhlink offers accuracy, power, and potential for revealing novel biological insights in gene regulation.

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