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Protocols for Endothelial Cell Isolation from Mouse Tissues: Small Intestine, Colon, Heart, and Liver

Overview
Journal STAR Protoc
Publisher Cell Press
Specialties Biology
Biomedical Engineering
Science
Date 2021 May 19
PMID 34007969
Citations 10
Authors
Liliana Sokol
Vincent Geldhof
Melissa Garcia-Caballero
Nadine V Conchinha
Sebastien J Dumas
Elda Meta
Laure-Anne Teuwen
Koen Veys
Rongyuan Chen
Lucas Treps
Mila Borri
Pauline de Zeeuw
Kim D Falkenberg
Charlotte Dubois
Magdalena Parys
Laura P M H de Rooij
Jermaine Goveia
Katerina Rohlenova
Luc Schoonjans
Mieke Dewerchin
Guy Eelen
Xuri Li
Joanna Kalucka
Peter Carmeliet
Affiliations
Soon will be listed here.
Abstract

Endothelial cells (ECs) from the small intestine, colon, liver, and heart have distinct phenotypes and functional adaptations that are dependent on their physiological environment. Gut ECs adapt to low oxygen, heart ECs to contractile forces, and liver ECs to low flow rates. Isolating high-purity ECs in sufficient quantities is crucial to study their functions. Here, we describe protocols combining magnetic and fluorescent activated cell sorting for rapid and reproducible EC purification from four adult murine tissues. For complete details on the use and execution of these protocols, please refer to Kalucka et al. (2020).

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