Single-cell Mapping Reveals New Markers and Functions of Lymphatic Endothelial Cells in Lymph Nodes

Overview

Journal PLoS Biol

Specialty Biology

Date 2020 Apr 7

PMID 32251437

Citations 75

Authors

Noriki Fujimoto

Yuliang He

Marco DAddio

Carlotta Tacconi

Michael Detmar

Lothar C Dieterich

Affiliations

Soon will be listed here.

Abstract

Lymph nodes (LNs) are highly organized secondary lymphoid organs that mediate adaptive immune responses to antigens delivered via afferent lymphatic vessels. Lymphatic endothelial cells (LECs) line intranodal lymphatic sinuses and organize lymph and antigen distribution. LECs also directly regulate T cells, mediating peripheral tolerance to self-antigens, and play a major role in many diseases, including cancer metastasis. However, little is known about the phenotypic and functional heterogeneity of LN LECs. Using single-cell RNA sequencing, we comprehensively defined the transcriptome of LECs in murine skin-draining LNs and identified new markers and functions of distinct LEC subpopulations. We found that LECs residing in the subcapsular sinus (SCS) have an unanticipated function in scavenging of modified low-density lipoprotein (LDL) and also identified a specific cortical LEC subtype implicated in rapid lymphocyte egress from LNs. Our data provide new, to our knowledge, insights into the diversity of LECs in murine LNs and a rich resource for future studies into the regulation of immune responses by LN LECs.

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