Identifying Novel Enhancer Elements with CRISPR-Based Screens

Overview

Journal ACS Chem Biol

Publisher American Chemical Society

Specialties Biochemistry
Biology

Date 2018 Jan 5

PMID 29300083

Citations 15

Authors

Jason C Klein

Wei Chen

Molly Gasperini

Jay Shendure

Affiliations

Soon will be listed here.

Abstract

Enhancers control the spatiotemporal expression of genes and are essential for encoding differentiation and development. Since their discovery more than three decades ago, researchers have largely studied enhancers removed from their genomic context. The recent adaptation of CRISPR/Cas9 to genome editing in higher organisms now allows researchers to perturb and test these elements in their genomic context, through both mutation and epigenetic modulation. In this Perspective, we discuss recent advances in scanning noncoding regions of the genome for enhancer activity using CRISPR-based tools.

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