Epstein-Barr Virus Induces Germinal Center Light Zone Chromatin Architecture and Promotes Survival Through Enhancer Looping at the Locus

Overview

Journal mBio

Publisher American Society for Microbiology

Specialty Microbiology

Date 2023 Dec 7

PMID 38059622

Authors

Joanne Dai

Elliott D SoRelle

Emma Heckenberg

Lingyun Song

Jana M Cable

Gregory E Crawford

Micah A Luftig

Affiliations

Soon will be listed here.

Abstract

Epstein-Barr virus has evolved with its human host leading to an intimate relationship where infection of antibody-producing B cells mimics the process by which these cells normally recognize foreign antigens and become activated. Virtually everyone in the world is infected by adulthood and controls this virus pushing it into life-long latency. However, immune-suppressed individuals are at high risk for EBV+ cancers. Here, we isolated B cells from tonsils and compare the underlying molecular genetic differences between these cells and those infected with EBV. We find similar regulatory mechanism for expression of an important cellular protein that enables B cells to survive in lymphoid tissue. These findings link an underlying relationship at the molecular level between EBV-infected B cells in vitro with normally activated B cells . Our studies also characterize the role of a key viral control mechanism for B cell survival involved in long-term infection.

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Epstein-Barr virus protein EBNA-LP engages YY1 through leucine-rich motifs to promote naïve B cell transformation.

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Epstein-Barr virus evades restrictive host chromatin closure by subverting B cell activation and germinal center regulatory loci.

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