Direct Inhibition of RNA Polymerase II Transcription by RECQL5

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2009 Jul 3

PMID 19570979

Citations 44

Authors

Ozan Aygun

Xiaohua Xu

Yilun Liu

Hidehisa Takahashi

Stephanie E Kong

Ronald C Conaway

Joan W Conaway

Jesper Q Svejstrup

Affiliations

Soon will be listed here.

Abstract

DNA helicases of the RECQ family are important for maintaining genome integrity, from bacteria to humans. Although progress has been made in understanding the biochemical role of some human RECQ helicases, that of RECQL5 remains elusive. We recently reported that RECQL5 interacts with RNA polymerase II (RNAPII), pointing to a role for the protein in transcription. Here, we show that RECQL5 inhibits both initiation and elongation in transcription assays reconstituted with highly purified general transcription factors and RNAPII. Such inhibition is not observed with the related, much more active RECQL1 helicase or with a version of RECQL5 that has normal helicase activity but is impaired in its ability to interact with RNAPII. Indeed, RECQL5 helicase activity is not required for inhibition. We discuss our findings in light of the fact that RECQ5(-/-) mice have elevated levels of DNA recombination and a higher incidence of cancer.

Citing Articles

Structural insights into transcriptional regulation by the helicase RECQL5.

Ariza A, Lue N, Grob P, Kaeser B, Fang J, Kassube S bioRxiv. 2025; .

PMID: 39975028 PMC: 11838285. DOI: 10.1101/2025.01.29.634372.

RNA biogenesis and RNA metabolism factors as R-loop suppressors: a hidden role in genome integrity.

Luna R, Gomez-Gonzalez B, Aguilera A Genes Dev. 2024; 38(11-12):504-527.

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Expression of human RECQL5 in Saccharomyces cerevisiae causes transcription defects and transcription-associated genome instability.

Lafuente-Barquero J, Svejstrup J, Luna R, Aguilera A Mol Genet Genomics. 2024; 299(1):59.

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ATM, KAP1 and the Epstein-Barr virus polymerase processivity factor direct traffic at the intersection of transcription and replication.

Xu H, Akinyemi I, Haley J, McIntosh M, Bhaduri-McIntosh S Nucleic Acids Res. 2023; 51(20):11104-11122.

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Understanding the Human RECQ5 Helicase-Connecting the Dots from DNA to Clinics.

Mo C, Shiozaki Y, Omabe K, Liu Y Cells. 2023; 12(16).

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