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Kotaro Nakanishi

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Articles 36
Citations 1314
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Recent Articles
1.
Zhang H, Sim G, Kehling A, Adhav V, Savidge A, Pastore B, et al.
Cell Rep . 2024 Oct; 43(10):114806. PMID: 39368090
TinyRNAs (tyRNAs) are ≤17-nt guide RNAs associated with Argonaute proteins (AGOs), and certain 14-nt cleavage-inducing tyRNAs (cityRNAs) catalytically activate human Argonaute3 (AGO3). We present the crystal structure of AGO3 in...
2.
Yang X, Shen Z, Wang C, Nakanishi K, Fu T
Cell . 2024 Aug; 187(19):5253-5266.e16. PMID: 39173632
Horizontal gene transfer is a key driver of bacterial evolution, but it also presents severe risks to bacteria by introducing invasive mobile genetic elements. To counter these threats, bacteria have...
3.
Yang X, Shen Z, Wang C, Nakanishi K, Fu T
bioRxiv . 2024 Jul; PMID: 39071313
Horizontal gene transfer is a key driver of bacterial evolution, but it also presents severe risks to bacteria by introducing invasive mobile genetic elements. To counter these threats, bacteria have...
4.
Nakanishi K, Kojima K, Sowa Y, Sudo Y
J Phys Chem B . 2024 Jul; 128(27):6509-6517. PMID: 38949422
Proton (H) motive force (PMF) serves as the energy source for the flagellar motor rotation, crucial for microbial motility. Here, to control PMF using light, we introduced light-driven inward and...
5.
Nakanishi K
J Biol Chem . 2023 Nov; 300(1):105499. PMID: 38029964
Argonaute (AGO) proteins in all three domains of life form ribonucleoprotein or deoxyribonucleoprotein complexes by loading a guide RNA or DNA, respectively. Since all AGOs retain a PIWI domain that...
6.
Sim G, Kehling A, Park M, Divoky C, Zhang H, Malhotra N, et al.
bioRxiv . 2023 Nov; PMID: 37961191
MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) are loaded into Argonaute (AGO) proteins, forming RNA-induced silencing complexes (RISCs). The assembly process establishes the seed, central, 3' supplementary, and tail regions...
7.
Sim G, Kehling A, Park M, Divoky C, Zhang H, Malhotra N, et al.
Sci Rep . 2023 Nov; 13(1):19761. PMID: 37957252
MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) are loaded into Argonaute (AGO) proteins, forming RNA-induced silencing complexes (RISCs). The assembly process establishes the seed, central, 3' supplementary, and tail regions...
8.
Shen Z, Yang X, Xia S, Huang W, Taylor D, Nakanishi K, et al.
Nature . 2023 Jul; 621(7977):154-161. PMID: 37494956
Although eukaryotic and long prokaryotic Argonaute proteins (pAgos) cleave nucleic acids, some short pAgos lack nuclease activity and hydrolyse NAD(P) to induce bacterial cell death. Here we present a hierarchical...
9.
Quevillon Huberdeau M, Shah V, Nahar S, Neumeier J, Houle F, Bruckmann A, et al.
Cell Rep . 2022 Dec; 41(11):111822. PMID: 36516777
Argonaute proteins are at the core of the microRNA-mediated gene silencing pathway essential for animals. In C. elegans, the microRNA-specific Argonautes ALG-1 and ALG-2 regulate multiple processes required for proper...
10.
Sim G, Kehling A, Park M, Secor J, Divoky C, Zhang H, et al.
Proc Natl Acad Sci U S A . 2022 Dec; 119(51):e2214335119. PMID: 36508664
MicroRNAs (miRNAs) are about 22-nucleotide (nt) noncoding RNAs forming the effector complexes with Argonaute (AGO) proteins to repress gene expression. Although tiny RNAs (tyRNAs) shorter than 19 nt have been...