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Barry L Wanner

Explore the profile of Barry L Wanner including associated specialties, affiliations and a list of published articles. Areas
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Articles 46
Citations 7303
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Recent Articles
1.
Murata M, Nakamura K, Kosaka T, Ota N, Osawa A, Muro R, et al.
Int J Mol Sci . 2021 Apr; 22(9). PMID: 33926096
The SOS response is induced upon DNA damage and the inhibition of Z ring formation by the product of the gene, which is one of the LexA-regulated genes, allows time...
2.
Oh D, Yu Y, Lee H, Jeon J, Wanner B, Ritchie K
PLoS One . 2018 May; 13(5):e0195887. PMID: 29771911
The spatial location of proteins in living cells can be critical for their function. For example, the E. coli chemotaxis machinery is localized to the cell poles. Here we describe...
3.
Ostrov N, Landon M, Guell M, Kuznetsov G, Teramoto J, Cervantes N, et al.
Science . 2016 Aug; 353(6301):819-22. PMID: 27540174
Recoding--the repurposing of genetic codons--is a powerful strategy for enhancing genomes with functions not commonly found in nature. Here, we report computational design, synthesis, and progress toward assembly of a...
4.
Nakahigashi K, Takai Y, Kimura M, Abe N, Nakayashiki T, Shiwa Y, et al.
DNA Res . 2016 Mar; 23(3):193-201. PMID: 27013550
Tetracycline-inhibited ribosome profiling (TetRP) provides a powerful new experimental tool for comprehensive genome-wide identification of translation initiation sites in bacteria. We validated TetRP by confirming the translation start sites of...
5.
Mori H, Takeuchi R, Otsuka Y, Bowden S, Yokoyama K, Muto A, et al.
Adv Exp Med Biol . 2015 Dec; 883:155-68. PMID: 26621467
E. coli has been a critically important model research organism for more than 50 years, particularly in molecular biology. In 1997, the E. coli draft genome sequence was published. Post-genomic...
6.
Mori H, Baba T, Yokoyama K, Takeuchi R, Nomura W, Makishi K, et al.
Methods Mol Biol . 2015 Feb; 1279:45-65. PMID: 25636612
Here we describe the systematic identification of single genes and gene pairs, whose knockout causes lethality in Escherichia coli K-12. During construction of precise single-gene knockout library of E. coli...
7.
Otsuka Y, Muto A, Takeuchi R, Okada C, Ishikawa M, Nakamura K, et al.
Nucleic Acids Res . 2014 Nov; 43(Database issue):D606-17. PMID: 25399415
Comprehensive experimental resources, such as ORFeome clone libraries and deletion mutant collections, are fundamental tools for elucidation of gene function. Data sets by omics analysis using these resources provide key...
8.
Conway T, Creecy J, Maddox S, Grissom J, Conkle T, Shadid T, et al.
mBio . 2014 Jul; 5(4):e01442-14. PMID: 25006232
We analyzed the transcriptome of Escherichia coli K-12 by strand-specific RNA sequencing at single-nucleotide resolution during steady-state (logarithmic-phase) growth and upon entry into stationary phase in glucose minimal medium. To...
9.
Takeuchi R, Tamura T, Nakayashiki T, Tanaka Y, Muto A, Wanner B, et al.
BMC Microbiol . 2014 Jun; 14:171. PMID: 24964927
Background: Precise quantitative growth measurements and detection of small growth changes in high-throughput manner is essential for fundamental studies of bacterial cell. However, an inherent tradeoff for measurement quality in...
10.
Yong H, Yamamoto N, Takeuchi R, Hsieh Y, Conrad T, Datsenko K, et al.
Genes Genet Syst . 2014 Jan; 88(4):233-40. PMID: 24463526
Genetic interaction networks are especially useful for functional assignment of genes and gaining new insights into the systems-level organization of the cell. While studying interactions of nonessential genes can be...