GenoBase: Comprehensive Resource Database of Escherichia Coli K-12
Overview
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Comprehensive experimental resources, such as ORFeome clone libraries and deletion mutant collections, are fundamental tools for elucidation of gene function. Data sets by omics analysis using these resources provide key information for functional analysis, modeling and simulation both in individual and systematic approaches. With the long-term goal of complete understanding of a cell, we have over the past decade created a variety of clone and mutant sets for functional genomics studies of Escherichia coli K-12. We have made these experimental resources freely available to the academic community worldwide. Accordingly, these resources have now been used in numerous investigations of a multitude of cell processes. Quality control is extremely important for evaluating results generated by these resources. Because the annotation has been changed since 2005, which we originally used for the construction, we have updated these genomic resources accordingly. Here, we describe GenoBase (http://ecoli.naist.jp/GB/), which contains key information about comprehensive experimental resources of E. coli K-12, their quality control and several omics data sets generated using these resources.
Global regulation via modulation of ribosome pausing by the ABC-F protein EttA.
Ousalem F, Ngo S, Oiffer T, Omairi-Nasser A, Hamon M, Monlezun L Nat Commun. 2024; 15(1):6314.
PMID: 39060293 PMC: 11282234. DOI: 10.1038/s41467-024-50627-z.
Bobonis J, Yang A, Voogdt C, Typas A Nat Protoc. 2024; 19(8):2231-2249.
PMID: 38724726 DOI: 10.1038/s41596-024-00988-y.
Genetic mutation in genome during adaptation to the murine intestine is optimized for the host diet.
Tsukimi T, Obana N, Shigemori S, Arakawa K, Miyauchi E, Yang J mSystems. 2024; 9(2):e0112323.
PMID: 38205998 PMC: 10878103. DOI: 10.1128/msystems.01123-23.
Soft-metal(loid)s induce protein aggregation in .
Cornejo F, Munoz-Villagran C, Luraschi R, Sandoval-Diaz M, Cancino C, Pugin B Front Microbiol. 2023; 14:1281058.
PMID: 38075883 PMC: 10699150. DOI: 10.3389/fmicb.2023.1281058.
Structural polymorphism and substrate promiscuity of a ribosome-associated molecular chaperone.
Huang C, Lai Y, Chen S, Ho M, Chiang Y, Hsu S Magn Reson (Gott). 2023; 2(1):375-386.
PMID: 37904759 PMC: 10539794. DOI: 10.5194/mr-2-375-2021.