V Kappa Gene Family in (Glu60 Ala30 Tyr10)n (GAT)-specific Antibodies That Express CGAT (or PGAT) Public Idiotypic Specificities. Protein and MRNA Sequencing of Eight Monoclonal V Kappa Chains

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1983 Nov 1

PMID 6415205

Citations 8

Authors

C Tonnelle

A Moulin

D Moinier

M Fougereau

Affiliations

Soon will be listed here.

Abstract

A large proportion of (Glu60 Ala30 Tyr10)n (GAT)-specific antibodies expresses public idiotypic specificities, termed CGAT (or pGAT), that require the presence of both the heavy and the light chains in order to be expressed. We report in this paper the complete sequence of eight V kappa regions pertaining to eight anti-GAT monoclonal antibodies derived from three strains of mice: BALB/c, DBA/2, and C57BL/6. The methodology used a combination of NH2-terminal amino acid and mRNA nucleotide sequencing. All eight sequences analyzed, although highly homologous and all pertaining to the same V kappa 1 subgroup, allowed definition of three germline genes that are likely to be present in all three strains of mice and also in NZB. It seems likely, however, that any given strain may not necessarily use all three genes for making anti-GAT antibodies. The search for structural correlates of idiotypes could not be framed in a simple picture, but our data suggest that similar idiotopes may result from different interacting primary structures, leading to structural homologies that should be visualized at three-dimensional level.

Citing Articles

The idiotypic network and the internal image: possible regulation of a germ-line network by paucigene encoded Ab2 (anti-idiotypic) antibodies in the GAT system.

Ollier P, Somme G, Theze J, Fougereau M EMBO J. 1985; 4(13B):3681-8.

PMID: 3937730 PMC: 554718. DOI: 10.1002/j.1460-2075.1985.tb04135.x.

Gene repertoire of the anti-poly(Glu60Ala30Tyr10) (GAT) immune response: comparison of VH, V kappa, and D regions used by anti-GAT antibodies and monoclonal antibodies produced after anti-idiotypic immunization.

Roth C, Somme G, Fougereau M, Theze J Proc Natl Acad Sci U S A. 1985; 82(14):4788-92.

PMID: 3927291 PMC: 390990. DOI: 10.1073/pnas.82.14.4788.

Variable region sequences of murine IgM anti-IgG monoclonal autoantibodies (rheumatoid factors). A structural explanation for the high frequency of IgM anti-IgG B cells.

Shlomchik M, Nemazee D, Sato V, Van Snick J, Carson D, WEIGERT M J Exp Med. 1986; 164(2):407-27.

PMID: 3088205 PMC: 2188225. DOI: 10.1084/jem.164.2.407.

Genetic basis for expression of the idiotypic network. One unique Ig VH germline gene accounts for the major family of Ab1 and Ab3 (Ab1') antibodies of the GAT system.

Schiff C, Milili M, Hue I, Rudikoff S, Fougereau M J Exp Med. 1986; 163(3):573-87.

PMID: 3005465 PMC: 2188062. DOI: 10.1084/jem.163.3.573.

Influence of the macromolecular form of a B cell epitope on the expression of antibody variable and constant region structure.

Fish S, Manser T J Exp Med. 1987; 166(3):711-24.

PMID: 2442288 PMC: 2188690. DOI: 10.1084/jem.166.3.711.

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