A New Mouse Myeloma Cell Line That Has Lost Immunoglobulin Expression but Permits the Construction of Antibody-secreting Hybrid Cell Lines

Overview

Journal J Immunol

Specialty Allergy & Immunology

Date 1979 Oct 1

PMID 113458

Citations 475

Authors

J F Kearney

A Radbruch

B Liesegang

K Rajewsky

Affiliations

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Abstract

We have isolated a subclone of the mouse myeloma cell line P3-X63-Ag8 that does not express immunoglobulin heavy or light chains. This clone X63-Ag8.653 can be used for efficient fusion with antibody-forming cells to obtain hybrid cell lines producing pure monoclonal antibodies. Screening of hybrid cell lines for specificity and immunoglobulin classes was done with a modified enzyme-linked immunosorbent assay.

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