Gene Repertoire of the Anti-poly(Glu60Ala30Tyr10) (GAT) Immune Response: Comparison of VH, V Kappa, and D Regions Used by Anti-GAT Antibodies and Monoclonal Antibodies Produced After Anti-idiotypic Immunization

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1985 Jul 1

PMID 3927291

Citations 3

Authors

C Roth

G Somme

M Fougereau

J Theze

Affiliations

Soon will be listed here.

Abstract

Eight monoclonal antibodies were selected from BALB/c mice immunized with two different monoclonal anti-idiotypic antibodies recognizing two discrete idiotopes characteristic of the anti-poly(Glu60Ala30Tyr10) (GAT) antibody response. These monoclonal antibodies were previously classified as Ab1 (anti-GAT-like) and Ab3 (anti-anti-idiotype) on the basis of expression of the public idiotypic specificity (p.GAT) studied with a xenogeneic serum, anti-GAT activity, and expression of various public idiotopes. All the heavy chain variable region (VH) sequences from Ab1 are nearly identical to the VH sequences of Ab1 anti-GAT monoclonal antibodies. The same type of results has been found with the Ab1 kappa light chain variable region (V kappa) sequences. Confirming our classification, Ab3 VH and V kappa sequences were found to be completely different from Ab1 VH and V kappa sequences. The Ab1 diversity (D) regions are different from one another and different from the D regions found on monoclonal anti-GAT antibodies but function similarly. These D regions are not simply derived from already described D genes. Finally, our results suggest that in the anti-GAT response VH and V kappa sequence are mainly responsible for idiotype expression.

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