The Idiotypic Network and the Internal Image: Possible Regulation of a Germ-line Network by Paucigene Encoded Ab2 (anti-idiotypic) Antibodies in the GAT System

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1985 Dec 30

PMID 3937730

Citations 14

Authors

P Ollier

G Somme

J Theze

M Fougereau

Affiliations

Soon will be listed here.

Abstract

Heavy and light chain variable regions from eight monoclonal Ab2 (anti-idiotypic) antibodies of the GAT antigen, a (Glu60 Ala30 Tyr10)n co-polymer, have been analyzed by direct mRNA sequencing. Three mAb2s were directed against private idiotopes and used various VH-D-JH and Vk-Jk combinations. By contrast, the five 'anti-public' mAb2 antibodies used a very restricted repertoire, including all gene segments. Interestingly, within their D regions, Glu-Glu-Tyr or Tyr-Tyr-Glu sequences were reminiscent of the original (GAT) antigen and may act as possible internal images. A striking observation was that two mAb2 antibodies shared the same V-D-J sequence although derived from separate fusions. As this D sequence, 33 nucleotides long, has not been described so far, it is suggested that it may be encoded by a new germ-line D gene, acting as a crucial regulatory element in a GAT germ-line idiotypic network. An alternative model that may lead to the construction of this D segment by 'odd' rearrangements from pre-existing already reported sequences is also presented.

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