Generation of Antibody Activity from Immunoglobulin Polypeptide Chains Produced in Escherichia Coli

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1984 Jun 1

PMID 6374653

Citations 33

Authors

S Cabilly

A D Riggs

H PANDE

J E Shively

W E Holmes

M Rey

L J Perry

R Wetzel

H L Heyneker

Affiliations

Soon will be listed here.

Abstract

Plasmids have been constructed that direct the synthesis in Escherichia coli of heavy chains and/or light chains of an anti-carcinoembryonic antigen (CEA) antibody. Another plasmid was constructed for expression of a truncated form of heavy chain (Fd' fragment) in E. coli. Functional CEA-binding activity was obtained by in vitro reconstitution in E. coli extracts of heavy chain or Fd' fragment mixed with extracts containing light chain.

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