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Phenotypic Expression in E. Coli of a DNA Sequence Coding for Mouse Dihydrofolate Reductase

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Journal Nature
Specialty Science
Date 1978 Oct 19
PMID 360074
Citations 144
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Abstract

The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the antimetabolic drug trimethoprim.

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