Accelerating Single-Cell Sequencing Data Analysis with SciDAP: A User-Friendly Approach

Overview

Journal Methods Mol Biol

Date 2025 Feb 3

PMID 39900764

Authors

Michael Kotliar

Andrey Kartashov

Artem Barski

Affiliations

Soon will be listed here.

Abstract

Single-cell (sc) RNA, ATAC, and Multiome sequencing became powerful tools for uncovering biological and disease mechanisms. Unfortunately, manual analysis of sc data presents multiple challenges due to large data volumes and complexity of configuration parameters. This complexity, as well as not being able to reproduce a computational environment, affects the reproducibility of analysis results. The Scientific Data Analysis Platform ( https://SciDAP.com ) allows biologists without computational expertise to analyze sequencing-based data using portable and reproducible pipelines written in Common Workflow Language (CWL). Our suite of computational pipelines addresses the most common needs in scRNA-Seq, scATAC-Seq and scMultiome data analysis. When executed on SciDAP, it offers a user-friendly alternative to manual data processing, eliminating the need for coding expertise. In this protocol, we describe the use of SciDAP to analyze scMultiome data. Similar approaches can be used for analysis of scRNA-Seq, scATAC-Seq and scVDJ-Seq datasets.

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