Recommendations for Performing, Interpreting and Reporting Hydrogen Deuterium Exchange Mass Spectrometry (HDX-MS) Experiments

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2019 Jun 29

PMID 31249422

Citations 290

Authors

Glenn R Masson

John E Burke

Natalie G Ahn

Ganesh S Anand

Christoph Borchers

Sebastien Brier

George M Bou-Assaf

John R Engen

S Walter Englander

Johan Faber

Rachel Garlish

Patrick R Griffin

Michael L Gross

Miklos Guttman

Yoshitomo Hamuro

Albert J R Heck

Damian Houde

Roxana E Iacob

Thomas J D Jorgensen

Igor A Kaltashov

Judith P Klinman

Lars Konermann

Petr Man

Leland Mayne

Bruce D Pascal

Dana Reichmann

Mark Skehel

Joost Snijder

Timothy S Strutzenberg

Eric S Underbakke

Cornelia Wagner

Thomas E Wales

Benjamin T Walters

David D Weis

Derek J Wilson

Patrick L Wintrode

Zhongqi Zhang

Jie Zheng

David C Schriemer

Kasper D Rand

Affiliations

Soon will be listed here.

Abstract

Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful biophysical technique being increasingly applied to a wide variety of problems. As the HDX-MS community continues to grow, adoption of best practices in data collection, analysis, presentation and interpretation will greatly enhance the accessibility of this technique to nonspecialists. Here we provide recommendations arising from community discussions emerging out of the first International Conference on Hydrogen-Exchange Mass Spectrometry (IC-HDX; 2017). It is meant to represent both a consensus viewpoint and an opportunity to stimulate further additions and refinements as the field advances.

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