Hydrogen Exchange Mass Spectrometry: Are We out of the Quicksand?

Overview

Journal J Am Soc Mass Spectrom

Specialty Chemistry

Date 2012 Apr 6

PMID 22476891

Citations 52

Authors

Roxana E Iacob

John R Engen

Affiliations

Soon will be listed here.

Abstract

Although the use of hydrogen exchange (HX) mass spectrometry (MS) to study proteins and protein conformation is now over 20 years old, the perception lingers that it still has "issues." Is this method, in fact, still in the quicksand with many remaining obstacles to overcome? We do not think so. This critical insight addresses the "issues" and explores several broad questions including, have the limitations of HX MS been surmounted and has HX MS achieved "indispensable" status in the pantheon of protein structural analysis tools.

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