Genome-scale Engineering of Saccharomyces Cerevisiae with Single-nucleotide Precision

Overview

Journal Nat Biotechnol

Specialty Biotechnology

Date 2018 May 8

PMID 29734295

Citations 66

Authors

Zehua Bao

Mohammad HamediRad

Pu Xue

Han Xiao

Ipek Tasan

Ran Chao

Jing Liang

Huimin Zhao

Affiliations

Soon will be listed here.

Abstract

We developed a CRISPR-Cas9- and homology-directed-repair-assisted genome-scale engineering method named CHAnGE that can rapidly output tens of thousands of specific genetic variants in yeast. More than 98% of target sequences were efficiently edited with an average frequency of 82%. We validate the single-nucleotide resolution genome-editing capability of this technology by creating a genome-wide gene disruption collection and apply our method to improve tolerance to growth inhibitors.

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