High-efficiency Yeast Transformation Using the LiAc/SS Carrier DNA/PEG Method

Overview

Journal Nat Protoc

Specialties Biology
Pathology
Science

Date 2007 Apr 3

PMID 17401334

Citations 1195

Authors

R Daniel Gietz

Robert H Schiestl

Affiliations

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Abstract

Here we describe a high-efficiency version of the lithium acetate/single-stranded carrier DNA/PEG method of transformation of Saccharomyces cerevisiae. This method currently gives the highest efficiency and yield of transformants, although a faster protocol is available for small number of transformations. The procedure takes up to 1.5 h, depending on the length of heat shock, once the yeast culture has been grown. This method is useful for most transformation requirements.

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