Rapid Profiling of a Microbial Genome Using Mixtures of Barcoded Oligonucleotides

Overview

Journal Nat Biotechnol

Specialty Biotechnology

Date 2010 Jul 20

PMID 20639866

Citations 113

Authors

Joseph R Warner

Philippa J Reeder

Anis Karimpour-Fard

Lauren B A Woodruff

Ryan T Gill

Affiliations

Soon will be listed here.

Abstract

A fundamental goal in biotechnology and biology is the development of approaches to better understand the genetic basis of traits. Here we report a versatile method, trackable multiplex recombineering (TRMR), whereby thousands of specific genetic modifications are created and evaluated simultaneously. To demonstrate TRMR, in a single day we modified the expression of >95% of the genes in Escherichia coli by inserting synthetic DNA cassettes and molecular barcodes upstream of each gene. Barcode sequences and microarrays were then used to quantify population dynamics. Within a week we mapped thousands of genes that affect E. coli growth in various media (rich, minimal and cellulosic hydrolysate) and in the presence of several growth inhibitors (beta-glucoside, D-fucose, valine and methylglyoxal). This approach can be applied to a broad range of traits to identify targets for future genome-engineering endeavors.

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