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SCANPY: Large-scale Single-cell Gene Expression Data Analysis

Overview
Journal Genome Biol
Specialties Biology
Genetics
Date 2018 Feb 8
PMID 29409532
Citations 2829
Authors
Affiliations
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Abstract

SCANPY is a scalable toolkit for analyzing single-cell gene expression data. It includes methods for preprocessing, visualization, clustering, pseudotime and trajectory inference, differential expression testing, and simulation of gene regulatory networks. Its Python-based implementation efficiently deals with data sets of more than one million cells ( https://github.com/theislab/Scanpy ). Along with SCANPY, we present ANNDATA, a generic class for handling annotated data matrices ( https://github.com/theislab/anndata ).

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References
1.
Angerer P, Haghverdi L, Buttner M, Theis F, Marr C, Buettner F . destiny: diffusion maps for large-scale single-cell data in R. Bioinformatics. 2015; 32(8):1241-3. DOI: 10.1093/bioinformatics/btv715. View

2.
Macosko E, Basu A, Satija R, Nemesh J, Shekhar K, Goldman M . Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets. Cell. 2015; 161(5):1202-1214. PMC: 4481139. DOI: 10.1016/j.cell.2015.05.002. View

3.
Amir E, Davis K, Tadmor M, Simonds E, Levine J, Bendall S . viSNE enables visualization of high dimensional single-cell data and reveals phenotypic heterogeneity of leukemia. Nat Biotechnol. 2013; 31(6):545-52. PMC: 4076922. DOI: 10.1038/nbt.2594. View

4.
Buettner F, Natarajan K, Casale F, Proserpio V, Scialdone A, Theis F . Computational analysis of cell-to-cell heterogeneity in single-cell RNA-sequencing data reveals hidden subpopulations of cells. Nat Biotechnol. 2015; 33(2):155-60. DOI: 10.1038/nbt.3102. View

5.
Wagner A, Regev A, Yosef N . Revealing the vectors of cellular identity with single-cell genomics. Nat Biotechnol. 2016; 34(11):1145-1160. PMC: 5465644. DOI: 10.1038/nbt.3711. View