Targeting the Lipid Metabolic Axis in Colorectal Cancer Progression by Therapeutic MiRNAs: MiR-19b-1 Role

Overview

Journal J Lipid Res

Publisher Elsevier

Specialty Biochemistry

Date 2017 Oct 28

PMID 29074607

Citations 34

Authors

Silvia Cruz-Gil

Ruth Sanchez-Martinez

Marta Gomez de Cedron

Roberto Martin-Hernandez

Teodoro Vargas

Susana Molina

Jesus Herranz

Alberto Davalos

Guillermo Reglero

Ana Ramirez de Molina

Affiliations

Soon will be listed here.

Abstract

An abnormal acyl-CoA synthetase/stearoyl-CoA desaturase (/) lipid network fuels colon cancer progression, endowing cells with invasive and migratory properties. Therapies against this metabolic network may be useful to improve clinical outcomes. Because micro-RNAs (miRNAs/miRs) are important epigenetic regulators, we investigated novel miRNAs targeting this pro-tumorigenic axis; hence to be used as therapeutic or prognostic miRNAs. Thirty-one putative common miRNAs were predicted to simultaneously target the three enzymes comprising the / network. Target validation by quantitative RT-PCR, Western blotting, and luciferase assays showed miR-544a, miR-142, and miR-19b-1 as major regulators of the metabolic axis, / Importantly, lower miR-19b-1 expression was associated with a decreased survival rate in colorectal cancer (CRC) patients, accordingly with / involvement in patient relapse. Finally, miR-19b-1 regulated the pro-tumorigenic axis, /, being able to inhibit invasion in colon cancer cells. Because its expression correlated with an increased survival rate in CRC patients, we propose miR-19b-1 as a potential noninvasive biomarker of disease-free survival and a promising therapeutic miRNA in CRC.

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