HiChIP: Efficient and Sensitive Analysis of Protein-directed Genome Architecture

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2016 Sep 20

PMID 27643841

Citations 596

Authors

Maxwell R Mumbach

Adam J Rubin

Ryan A Flynn

Chao Dai

Paul A Khavari

William J Greenleaf

Howard Y Chang

Affiliations

Soon will be listed here.

Abstract

Genome conformation is central to gene control but challenging to interrogate. Here we present HiChIP, a protein-centric chromatin conformation method. HiChIP improves the yield of conformation-informative reads by over 10-fold and lowers the input requirement over 100-fold relative to that of ChIA-PET. HiChIP of cohesin reveals multiscale genome architecture with greater signal-to-background ratios than those of in situ Hi-C.

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