Noninvasive Detection of Inflammatory Changes in White Adipose Tissue by Label-Free Raman Spectroscopy

Overview

Journal Anal Chem

Specialty Chemistry

Date 2016 Jan 12

PMID 26752499

Citations 8

Authors

Abigail S Haka

Erika Sue

Chi Zhang

Priya Bhardwaj

Joshua Sterling

Cassidy Carpenter

Madeline Leonard

Maryem Manzoor

Jeanne Walker

Jose O Aleman

Daniel Gareau

Peter R Holt

Jan L Breslow

Xi Kathy Zhou

Dilip Giri

Monica Morrow

Neil Iyengar

Ishan Barman

Clifford A Hudis

Andrew J Dannenberg

Affiliations

Soon will be listed here.

Abstract

White adipose tissue inflammation (WATi) has been linked to the pathogenesis of obesity-related diseases, including type 2 diabetes, cardiovascular disease, and cancer. In addition to the obese, a substantial number of normal and overweight individuals harbor WATi, putting them at increased risk for disease. We report the first technique that has the potential to detect WATi noninvasively. Here, we used Raman spectroscopy to detect WATi with excellent accuracy in both murine and human tissues. This is a potentially significant advance over current histopathological techniques for the detection of WATi, which rely on tissue excision and, therefore, are not practical for assessing disease risk in the absence of other identifying factors. Importantly, we show that noninvasive Raman spectroscopy can diagnose WATi in mice. Taken together, these results demonstrate the potential of Raman spectroscopy to provide objective risk assessment for future cardiometabolic complications in both normal weight and overweight/obese individuals.

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