Condensin Targets and Reduces Unwound DNA Structures Associated with Transcription in Mitotic Chromosome Condensation

Overview

Journal Nat Commun

Specialty Biology

Date 2015 Jul 24

PMID 26204128

Citations 66

Authors

Takashi Sutani

Toyonori Sakata

Ryuichiro Nakato

Koji Masuda

Mai Ishibashi

Daisuke Yamashita

Yutaka Suzuki

Tatsuya Hirano

Masashige Bando

Katsuhiko Shirahige

Affiliations

Soon will be listed here.

Abstract

Chromosome condensation is a hallmark of mitosis in eukaryotes and is a prerequisite for faithful segregation of genetic material to daughter cells. Here we show that condensin, which is essential for assembling condensed chromosomes, helps to preclude the detrimental effects of gene transcription on mitotic condensation. ChIP-seq profiling reveals that the fission yeast condensin preferentially binds to active protein-coding genes in a transcription-dependent manner during mitosis. Pharmacological and genetic attenuation of transcription largely rescue bulk chromosome segregation defects observed in condensin mutants. We also demonstrate that condensin is associated with and reduces unwound DNA segments generated by transcription, providing a direct link between an in vitro activity of condensin and its in vivo function. The human condensin isoform condensin I also binds to unwound DNA regions at the transcription start sites of active genes, implying that our findings uncover a fundamental feature of condensin complexes.

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