Topoisomerases I and II Facilitate Condensin DC Translocation to Organize and Repress X Chromosomes in C. elegans

Overview

Journal Mol Cell

Publisher Cell Press

Specialty Cell Biology

Date 2022 Oct 27

PMID 36302374

Authors

Ana Karina Morao

Jun Kim

Daniel Obaji

Siyu Sun

Sevinc Ercan

Affiliations

Soon will be listed here.

Abstract

Condensins are evolutionarily conserved molecular motors that translocate along DNA and form loops. To address how DNA topology affects condensin translocation, we applied auxin-inducible degradation of topoisomerases I and II and analyzed the binding and function of an interphase condensin that mediates X chromosome dosage compensation in C. elegans. TOP-2 depletion reduced long-range spreading of condensin-DC (dosage compensation) from its recruitment sites and shortened 3D DNA contacts measured by Hi-C. TOP-1 depletion did not affect long-range spreading but resulted in condensin-DC accumulation within expressed gene bodies. Both TOP-1 and TOP-2 depletion resulted in X chromosome derepression, indicating that condensin-DC translocation at both scales is required for its function. Together, the distinct effects of TOP-1 and TOP-2 suggest two distinct modes of condensin-DC association with chromatin: long-range DNA loop extrusion that requires decatenation/unknotting of DNA and short-range translocation across genes that requires resolution of transcription-induced supercoiling.

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