Profiling a Single-stranded DNA Region Within an RDNA Segment That Affects the Loading of Bacterial Condensin

Overview

Journal iScience

Publisher Cell Press

Date 2022 Nov 28

PMID 36437875

Authors

Koichi Yano

Hideki Noguchi

Hironori Niki

Affiliations

Soon will be listed here.

Abstract

Bacterial condensin preferentially loads onto single-stranded DNA (ssDNA) and onto rDNA to support proper chromosome compaction. Thus, the actively transcribing rDNA would provide the ssDNA region for the loading of bacterial condensin. We attempted to detect the ssDNA region in the gene . Non-denaturing sodium bisulfite treatment catalyzed the conversion of cytosines to thymines (CT-conversion) at the melted DNA of a genome. Using next-generation sequencing, we generated an average of 11,000 reads covering each cytosine on the rDNA segment. In principle, the CT-conversion rate is an accurate guide to detect ssDNA segment. We detected multiple ssDNA segments throughout the rDNA. The deletion mutations of the rDNA hindered the ssDNA formation at the 100-500 bp segment downstream of the promoter. These data support the idea that the ssDNA segment plays a crucial role as the condensin-loading site and suggest the mechanism of condensin loading onto rDNA.

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