A Comprehensive Molecular Survey of Echinococcus Granulosus in Formalin-fixed Paraffin-embedded Tissues in Human Isolates in Turkey

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2011 Feb 3

PMID 21286751

Citations 23

Authors

Sami Simsek

Mustafa Kaplan

Ibrahim Hanifi Ozercan

Affiliations

Soon will be listed here.

Abstract

Cystic echinococcosis (CE) due to the formation of a hydatic cyst is a disease commonly seen in humans and animals that can be mortal sometimes. This disease, which is present in many countries around the world, constitutes a great problem for public health and the economy. The aim of this study was to identify Echinococcus granulosus genotypes in formalin-fixed, paraffin-embedded tissues. Tissue samples from 70 human patients with histologically confirmed echinococcosis were analysed by direct PCR of the 12S rRNA gene and by DNA sequence analysis of the CO1 gene of E. granulosus. Of the 70 samples, 29 (41.6%) could be genotypically characterized. Specifically, 26 of 70 were positive by direct G1-3 PCR of the 12S rRNA gene, two of which were identified as G1 by additional CO1 gene sequencing. All the 44 unidentified samples underwent CO1 sequencing, which yielded one G3 and two G6 records, while the remaining 41 samples gave no or inconclusive results. In conclusion, the results from the analysis of human isolates of E. granulosus confirmed the occurrence of G1, G3 and G6 genotypes in Turkey and indicated G1/G3 cluster (E. granulosus sensu stricto) as the predominant genotype.

Citing Articles

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References

Manterola C, Benavente F, Melo A, Vial M, Roa J . Description of Echinococcus granulosus genotypes in human hydatidosis in a region of southern Chile. Parasitol Int. 2008; 57(3):342-6. DOI: 10.1016/j.parint.2008.02.005. View

Thompson R, Lymbery A . Echinococcus: biology and strain variation. Int J Parasitol. 1990; 20(4):457-70. DOI: 10.1016/0020-7519(90)90193-q. View

Rosenzvit M, Zhang L, Kamenetzky L, Canova S, Guarnera E, McManus D . Genetic variation and epidemiology of Echinococcus granulosus in Argentina. Parasitology. 1999; 118 ( Pt 5):523-30. DOI: 10.1017/s0031182099004035. View

Shi S, Cote R, Wu L, Liu C, Datar R, Shi Y . DNA extraction from archival formalin-fixed, paraffin-embedded tissue sections based on the antigen retrieval principle: heating under the influence of pH. J Histochem Cytochem. 2002; 50(8):1005-11. DOI: 10.1177/002215540205000802. View

Thompson R, Boxell A, Ralston B, Constantine C, Hobbs R, Shury T . Molecular and morphological characterization of Echinococcus in cervids from North America. Parasitology. 2005; 132(Pt 3):439-47. DOI: 10.1017/S0031182005009170. View

Nakao M, Yanagida T, Okamoto M, Knapp J, Nkouawa A, Sako Y . State-of-the-art Echinococcus and Taenia: phylogenetic taxonomy of human-pathogenic tapeworms and its application to molecular diagnosis. Infect Genet Evol. 2010; 10(4):444-52. DOI: 10.1016/j.meegid.2010.01.011. View

Schneider R, Gollackner B, Edel B, Schmid K, Wrba F, Tucek G . Development of a new PCR protocol for the detection of species and genotypes (strains) of Echinococcus in formalin-fixed, paraffin-embedded tissues. Int J Parasitol. 2008; 38(8-9):1065-71. DOI: 10.1016/j.ijpara.2007.11.008. View

Snabel V, DAmelio S, Nakao M, Romig T, Yolasigmaz A, Gunes K . Cystic echinococcosis in Turkey: genetic variability and first record of the pig strain (G7) in the country. Parasitol Res. 2009; 105(1):145-54. DOI: 10.1007/s00436-009-1376-2. View

Sharbatkhori M, Harandi M, Mirhendi H, Hajialilo E, Kia E . Sequence analysis of cox1 and nad1 genes in Echinococcus granulosus G3 genotype in camels (Camelus dromedarius) from central Iran. Parasitol Res. 2010; 108(3):521-7. DOI: 10.1007/s00436-010-2092-7. View

10.

Simsek S, Balkaya I, Koroglu E . Epidemiological survey and molecular characterization of Echinococcus granulosus in cattle in an endemic area of eastern Turkey. Vet Parasitol. 2010; 172(3-4):347-9. DOI: 10.1016/j.vetpar.2010.05.016. View

11.

Kamenetzky L, Canova S, Guarnera E, Rosenzvit M . Echinococcus granulosus: DNA extraction from germinal layers allows strain determination in fertile and nonfertile hydatid cysts. Exp Parasitol. 2000; 95(2):122-7. DOI: 10.1006/expr.2000.4518. View

12.

Simsek S, Eroksuz Y . Occurrence and molecular characterization of Echinococcus granulosus in Turkish mouflon (Ovis gmelinii anatolica). Acta Trop. 2008; 109(2):167-9. DOI: 10.1016/j.actatropica.2008.10.008. View

13.

Dinkel A, Njoroge E, Zimmermann A, Walz M, Zeyhle E, Elmahdi I . A PCR system for detection of species and genotypes of the Echinococcus granulosus-complex, with reference to the epidemiological situation in eastern Africa. Int J Parasitol. 2004; 34(5):645-53. DOI: 10.1016/j.ijpara.2003.12.013. View

14.

Lavikainen A, Lehtinen M, Meri T, Hirvela-Koski V, Meri S . Molecular genetic characterization of the Fennoscandian cervid strain, a new genotypic group (G10) of Echinococcus granulosus. Parasitology. 2003; 127(Pt 3):207-15. DOI: 10.1017/s0031182003003780. View

15.

Nakao M, McManus D, Schantz P, Craig P, Ito A . A molecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes. Parasitology. 2006; 134(Pt 5):713-22. DOI: 10.1017/S0031182006001934. View

16.

Obwaller A, Schneider R, Walochnik J, Gollackner B, Deutz A, Janitschke K . Echinococcus granulosus strain differentiation based on sequence heterogeneity in mitochondrial genes of cytochrome c oxidase-1 and NADH dehydrogenase-1. Parasitology. 2004; 128(Pt 5):569-75. DOI: 10.1017/s0031182004004871. View

17.

Utuk A, Simsek S, Koroglu E, McManus D . Molecular genetic characterization of different isolates of Echinococcus granulosus in east and southeast regions of Turkey. Acta Trop. 2008; 107(2):192-4. DOI: 10.1016/j.actatropica.2008.05.026. View

18.

Vural G, Baca A, Gauci C, Bagci O, Gicik Y, Lightowlers M . Variability in the Echinococcus granulosus cytochrome C oxidase 1 mitochondrial gene sequence from livestock in Turkey and a re-appraisal of the G1-3 genotype cluster. Vet Parasitol. 2008; 154(3-4):347-50. DOI: 10.1016/j.vetpar.2008.03.020. View

19.

Thompson R, Lymbery A, Constantine C . Variation in Echinococcus: towards a taxonomic revision of the genus. Adv Parasitol. 1995; 35:145-76. DOI: 10.1016/s0065-308x(08)60071-8. View

20.

Moks E, Jogisalu I, Valdmann H, Saarma U . First report of Echinococcus granulosus G8 in Eurasia and a reappraisal of the phylogenetic relationships of 'genotypes' G5-G10. Parasitology. 2008; 135(5):647-54. DOI: 10.1017/S0031182008004198. View