Investigation of the Relationship Between CE Cyst Characteristics and Genetic Diversity of in Humans from Turkey

Overview

Journal Parasitology

Specialty Parasitology

Date 2020 Aug 18

PMID 32799957

Citations 4

Authors

Serra Orsten

Turkmen Ciftci

Aynur Azizova

Gokhan Yuce

Aycan Uysal

Cetin Imamoglu

Ergun Karaagaoglu

Devrim Akinci

Yakut Akyon

Adriano Casulli

Okan Akhan

Affiliations

Soon will be listed here.

Abstract

Cystic echinococcosis (CE) is one of the most common zoonotic diseases worldwide, particularly in rural areas. This study aimed at the identification of the genotype/species belonging to Echinococcus granulosus sensu lato (s.l.) specimens in retrieved percutaneously from the human host and to investigate their relationship with cyst characteristics. The genetic identification of cyst material was performed by mt-CO1 gene polymerase chain reaction, and confirmed via sequencing. A total of 110 CE cysts were identified as E. granulosus s.l. In detail, 104 belonged to E. granulosus sensu stricto (G1 and G3) and six isolates were in the E. canadensis cluster (G6/7). All clusters were tested for the relationship between demographics, cyst features and genetic diversity. The relationship between genetic variation and certain clinical characteristics such as cyst volume and location were statistically significant for G6/7 cluster. Further studies are required with a larger sample set to investigate the relationship between the genetic variability of E. granulosus s.l. and cyst features.

Citing Articles

Assessing the Potential Apoptotic Effects of Different Hydatid Cyst Fluids on Human Healthy Hepatocytes and Hepatocellular Carcinoma Cells.

Baysal I, Orsten S, Cengiz G, Unal E, Dogrul A, Ciftci T Acta Parasitol. 2024; 69(1):700-709.

PMID: 38372909 PMC: 11001659. DOI: 10.1007/s11686-024-00797-z.

Genotyping of Human Isolates Obtained from the Puncture, Aspiration, Injection, and Re-Aspiration (PAIR) Process in Türkiye.

Akkas O, Ozgokce M, Aydemir S, Dundar I, Ekici A Iran J Parasitol. 2024; 18(4):427-434.

PMID: 38169642 PMC: 10758082. DOI: 10.18502/ijpa.v18i4.14242.

Differences in clinical aspects of human cystic echinococcosis caused by and the G6 genotype in Neuquén, Argentina.

Debiaggi M, Alvarez Rojas C, Lazzarini L, Calfunao D, Titanti P, Calanni L Parasitology. 2023; 150(7):599-605.

PMID: 37203451 PMC: 10260295. DOI: 10.1017/S0031182023000264.

Human and animal cystic echinococcosis in Konya, Turkey: molecular identification and the first report of E. equinus from human host in Turkey.

Macin S, Orsten S, Samadzade R, Colak B, Cebeci H, Findik D Parasitol Res. 2021; 120(2):563-568.

PMID: 33462709 PMC: 7813620. DOI: 10.1007/s00436-021-07050-w.

References

Librado P, Rozas J . DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics. 2009; 25(11):1451-2. DOI: 10.1093/bioinformatics/btp187. View

Thompson R . The taxonomy, phylogeny and transmission of Echinococcus. Exp Parasitol. 2008; 119(4):439-446. DOI: 10.1016/j.exppara.2008.04.016. View

Altintas N . Past to present: echinococcosis in Turkey. Acta Trop. 2003; 85(2):105-12. DOI: 10.1016/s0001-706x(02)00213-9. View

Salzburger W, Ewing G, von Haeseler A . The performance of phylogenetic algorithms in estimating haplotype genealogies with migration. Mol Ecol. 2011; 20(9):1952-63. DOI: 10.1111/j.1365-294X.2011.05066.x. View

Romig T, Deplazes P, Jenkins D, Giraudoux P, Massolo A, Craig P . Ecology and Life Cycle Patterns of Echinococcus Species. Adv Parasitol. 2017; 95:213-314. DOI: 10.1016/bs.apar.2016.11.002. View

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S . MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol. 2013; 30(12):2725-9. PMC: 3840312. DOI: 10.1093/molbev/mst197. View

Nakao M, Yanagida T, Konyaev S, Lavikainen A, Odnokurtsev V, Zaikov V . Mitochondrial phylogeny of the genus Echinococcus (Cestoda: Taeniidae) with emphasis on relationships among Echinococcus canadensis genotypes. Parasitology. 2013; 140(13):1625-36. DOI: 10.1017/S0031182013000565. View

Casulli A, Siles-Lucas M, Tamarozzi F . Echinococcus granulosus sensu lato. Trends Parasitol. 2019; 35(8):663-664. DOI: 10.1016/j.pt.2019.05.006. View

Orsten S, Boufana B, Ciftci T, Akinci D, Karaagaoglu E, Ozkuyumcu C . Human cystic echinococcosis in Turkey: a preliminary study on DNA polymorphisms of hydatid cysts removed from confirmed patients. Parasitol Res. 2018; 117(4):1257-1263. DOI: 10.1007/s00436-018-5807-9. View

10.

Wen H, Vuitton L, Tuxun T, Li J, Vuitton D, Zhang W . Echinococcosis: Advances in the 21st Century. Clin Microbiol Rev. 2019; 32(2). PMC: 6431127. DOI: 10.1128/CMR.00075-18. View

11.

Erdogan E, Ozkan B, Mutlu F, Karaca S, Sahin I . [Molecular characterization of Echinococcus granulosus isolates obtained from different hosts]. Mikrobiyol Bul. 2017; 51(1):79-86. DOI: 10.5578/mb.45452. View

12.

LARKIN M, Blackshields G, Brown N, Chenna R, McGettigan P, McWilliam H . Clustal W and Clustal X version 2.0. Bioinformatics. 2007; 23(21):2947-8. DOI: 10.1093/bioinformatics/btm404. View

13.

Deplazes P, Rinaldi L, Alvarez Rojas C, Torgerson P, Harandi M, Romig T . Global Distribution of Alveolar and Cystic Echinococcosis. Adv Parasitol. 2017; 95:315-493. DOI: 10.1016/bs.apar.2016.11.001. View

14.

Nakao M, Yanagida T, Okamoto M, Knapp J, Nkouawa A, Sako Y . State-of-the-art Echinococcus and Taenia: phylogenetic taxonomy of human-pathogenic tapeworms and its application to molecular diagnosis. Infect Genet Evol. 2010; 10(4):444-52. DOI: 10.1016/j.meegid.2010.01.011. View

15.

Sadjjadi S, Mikaeili F, Karamian M, Maraghi S, Sadjjadi F, Shariat-Torbaghan S . Evidence that the Echinococcus granulosus G6 genotype has an affinity for the brain in humans. Int J Parasitol. 2013; 43(11):875-7. DOI: 10.1016/j.ijpara.2013.06.008. View

16.

Hassan Z, Meerkhan A, Boufana B, Hama A, Ahmed B, Mero W . Two haplotype clusters of Echinococcus granulosus sensu stricto in northern Iraq (Kurdistan region) support the hypothesis of a parasite cradle in the Middle East. Acta Trop. 2017; 172:201-207. DOI: 10.1016/j.actatropica.2017.04.028. View

17.

Bakal U, Simsek S, Kazez A . Surgical and Molecular Evaluation of Pediatric Hydatid Cyst Cases in Eastern Turkey. Korean J Parasitol. 2016; 53(6):785-8. PMC: 4725236. DOI: 10.3347/kjp.2015.53.6.785. View

18.

Snabel V, DAmelio S, Nakao M, Romig T, Yolasigmaz A, Gunes K . Cystic echinococcosis in Turkey: genetic variability and first record of the pig strain (G7) in the country. Parasitol Res. 2009; 105(1):145-54. DOI: 10.1007/s00436-009-1376-2. View

19.

Romig T, Ebi D, Wassermann M . Taxonomy and molecular epidemiology of Echinococcus granulosus sensu lato. Vet Parasitol. 2015; 213(3-4):76-84. DOI: 10.1016/j.vetpar.2015.07.035. View

20.

Nakao M, Sako Y, Yokoyama N, Fukunaga M, Ito A . Mitochondrial genetic code in cestodes. Mol Biochem Parasitol. 2001; 111(2):415-24. DOI: 10.1016/s0166-6851(00)00334-0. View