Sequence Analysis of Cox1 and Nad1 Genes in Echinococcus Granulosus G3 Genotype in Camels (Camelus Dromedarius) from Central Iran

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2010 Oct 6

PMID 20922418

Citations 50

Authors

Mitra Sharbatkhori

Majid Fasihi Harandi

Hossein Mirhendi

Elham Hajialilo

Eshrat Beigom Kia

Affiliations

Soon will be listed here.

Abstract

Nineteen hydatid cyst isolates collected from camels in central Iran were subjected to sequences analysis of mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) genes. A consensus sequence obtained containing 366 nucleotides for cox1 and 471 nucleotides for nad1 genes. Overall, the camel isolates indicated five different sequences in cox1 and nine in nad1 genes. The sequences analysis indicated that 26.3%, 42.1%, and 31.6% of isolates belonging to G1, G3, and G6 genotypes of Echinococcus granulosus, respectively. The isolates with G3 genotype indicated one cox1 sequence having 100% homology with reference G3 sequence (AN: M84663) and two different nad1 sequences, one having 100% homology with reference G3 sequence (AN: AJ237634) and the other with a silent mutation (G to A) in position 279. The presence of G3 genotype (buffalo strain) of E. granulosus as dominant genotype in camels is emphasized. As G3 genotype has formerly been reported in human, the epidemiological role of camels is warranted in future surveys.

Citing Articles

Genetic profile of isolated from the livestock and human in northwest Iran.

Arabloo S, Johkool M, Mohammadi M, Mohammadzadeh A, Mohammadi D, Harandi M J Parasit Dis. 2025; 49(1):142-148.

PMID: 39975620 PMC: 11832864. DOI: 10.1007/s12639-024-01738-3.

Epidemiological and genotypic assessment of cystic echinococcosis in ruminant populations of Northern Punjab, Pakistan: a neglected zoonotic disease.

Khan S, Cable J, Masud N, Hailer F, Younus M, Hussain N Parasitol Res. 2025; 124(1):7.

PMID: 39821816 PMC: 11739240. DOI: 10.1007/s00436-025-08451-x.

Phylogeography, Genetic Diversity and Population Structure of Sensu Stricto Inferred by Mitochondrial DNA Markers between Southeast of Iran and Pakistan.

Anvari D, Gholami S, Spotin A, Hosseini S, Daryani A, Sarvi S Iran J Parasitol. 2024; 19(2):140-152.

PMID: 39011532 PMC: 11246203. DOI: 10.18502/ijpa.v19i2.15850.

Prevalence, risk factors and first record of mitochondrial 1 gene-based molecular characterization of from Pakistan.

Alvi M, Alshammari A, Asghar F, Ali R, Li L, Saqib M Front Vet Sci. 2022; 9:1018854.

PMID: 36478949 PMC: 9719931. DOI: 10.3389/fvets.2022.1018854.

Prevalence of Cystic Echinococcosis Genotypes in Iranian Animals: A Systematic Review and Meta-Analysis.

Khodashenas S, Akbari M, Beiranvand R, Didehdar M, Shabani M, Iravani P J Parasitol Res. 2022; 2022:8197741.

PMID: 36312205 PMC: 9605837. DOI: 10.1155/2022/8197741.

References

Zhang L, Eslami A, Hosseini S, McManus D . Indication of the presence of two distinct strains of Echinococcus granulosus in Iran by mitochondrial DNA markers. Am J Trop Med Hyg. 1998; 59(1):171-4. DOI: 10.4269/ajtmh.1998.59.171. View

Latif A, Tanveer A, Maqbool A, Siddiqi N, Kyaw-Tanner M, Traub R . Morphological and molecular characterisation of Echinococcus granulosus in livestock and humans in Punjab, Pakistan. Vet Parasitol. 2010; 170(1-2):44-9. DOI: 10.1016/j.vetpar.2010.02.003. View

McManus D, Zhang W, Li J, Bartley P . Echinococcosis. Lancet. 2003; 362(9392):1295-304. DOI: 10.1016/S0140-6736(03)14573-4. View

Fasihi Harandi M, Hobbs R, Adams P, Mobedi I, Morgan-Ryan U, Thompson R . Molecular and morphological characterization of Echinococcus granulosus of human and animal origin in Iran. Parasitology. 2002; 125(Pt 4):367-73. DOI: 10.1017/s0031182002002172. View

Capuano F, Rinaldi L, Maurelli M, Perugini A, Veneziano V, Garippa G . Cystic echinococcosis in water buffaloes: epidemiological survey and molecular evidence of ovine (G1) and buffalo (G3) strains. Vet Parasitol. 2006; 137(3-4):262-8. DOI: 10.1016/j.vetpar.2006.01.016. View

Gasser R, Zhu X, Woods W . Genotyping Taenia tapeworms by single-strand conformation polymorphism of mitochondrial DNA. Electrophoresis. 1999; 20(14):2834-7. DOI: 10.1002/(SICI)1522-2683(19991001)20:14<2834::AID-ELPS2834>3.0.CO;2-F. View

Rinaldi L, Maurelli M, Veneziano V, Capuano F, Perugini A, Cringoli S . The role of cattle in the epidemiology of Echinococcus granulosus in an endemic area of southern Italy. Parasitol Res. 2008; 103(1):175-9. DOI: 10.1007/s00436-008-0948-x. View

Lavikainen A, Haukisalmi V, Lehtinen M, Henttonen H, Oksanen A, Meri S . A phylogeny of members of the family Taeniidae based on the mitochondrial cox1 and nad1 gene data. Parasitology. 2008; 135(12):1457-67. DOI: 10.1017/S003118200800499X. View

Bardonnet K, Benchikh-Elfegoun M, Bart J, Harraga S, Hannache N, Haddad S . Cystic echinococcosis in Algeria: cattle act as reservoirs of a sheep strain and may contribute to human contamination. Vet Parasitol. 2003; 116(1):35-44. DOI: 10.1016/s0304-4017(03)00255-3. View

10.

Thompson R . The taxonomy, phylogeny and transmission of Echinococcus. Exp Parasitol. 2008; 119(4):439-446. DOI: 10.1016/j.exppara.2008.04.016. View

11.

Moks E, Jogisalu I, Valdmann H, Saarma U . First report of Echinococcus granulosus G8 in Eurasia and a reappraisal of the phylogenetic relationships of 'genotypes' G5-G10. Parasitology. 2008; 135(5):647-54. DOI: 10.1017/S0031182008004198. View

12.

Scott J, Stefaniak J, Pawlowski Z, McManus D . Molecular genetic analysis of human cystic hydatid cases from Poland: identification of a new genotypic group (G9) of Echinococcus granulosus. Parasitology. 1997; 114 ( Pt 1):37-43. DOI: 10.1017/s0031182096008062. View

13.

Tashani O, Zhang L, Boufana B, Jegi A, McManus D . Epidemiology and strain characteristics of Echinococcus granulosus in the Benghazi area of eastern Libya. Ann Trop Med Parasitol. 2002; 96(4):369-81. DOI: 10.1179/000349802125000952. View

14.

Pednekar R, Gatne M, Thompson R, Traub R . Molecular and morphological characterisation of Echinococcus from food producing animals in India. Vet Parasitol. 2009; 165(1-2):58-65. DOI: 10.1016/j.vetpar.2009.06.021. View

15.

Manterola C, Benavente F, Melo A, Vial M, Roa J . Description of Echinococcus granulosus genotypes in human hydatidosis in a region of southern Chile. Parasitol Int. 2008; 57(3):342-6. DOI: 10.1016/j.parint.2008.02.005. View

16.

Dinkel A, Njoroge E, Zimmermann A, Walz M, Zeyhle E, Elmahdi I . A PCR system for detection of species and genotypes of the Echinococcus granulosus-complex, with reference to the epidemiological situation in eastern Africa. Int J Parasitol. 2004; 34(5):645-53. DOI: 10.1016/j.ijpara.2003.12.013. View

17.

Lavikainen A, Lehtinen M, Meri T, Hirvela-Koski V, Meri S . Molecular genetic characterization of the Fennoscandian cervid strain, a new genotypic group (G10) of Echinococcus granulosus. Parasitology. 2003; 127(Pt 3):207-15. DOI: 10.1017/s0031182003003780. View

18.

Bardonnet K, Piarroux R, Dia L, Schneegans F, Beurdeley A, Godot V . Combined eco-epidemiological and molecular biology approaches to assess Echinococcus granulosus transmission to humans in Mauritania: occurrence of the 'camel' strain and human cystic echinococcosis. Trans R Soc Trop Med Hyg. 2002; 96(4):383-6. DOI: 10.1016/s0035-9203(02)90369-x. View

19.

Zhang L, Joshi D, McManus D . Three genotypes of Echinococcus granulosus identified in Nepal using mitochondrial DNA markers. Trans R Soc Trop Med Hyg. 2000; 94(3):258-60. DOI: 10.1016/s0035-9203(00)90313-4. View

20.

Bowles J, McManus D . Genetic characterization of the Asian Taenia, a newly described taeniid cestode of humans. Am J Trop Med Hyg. 1994; 50(1):33-44. View