Molecular Diagnosis of Echinococcosis in Patients Based on Frozen Paraffin Tissue Samples and Fixed Formalin and Hydatid Cysts Isolated from Livestock in a Slaughterhouse

Overview

Journal Trop Parasitol

Specialty Parasitology

Date 2024 Mar 6

PMID 38444797

Authors

Behjat Rahpima

Mansour Dabirzadeh

Affiliations

Soon will be listed here.

Abstract

Background: Various genotypes of have been studied in high-disease-risk areas and identified as causative agents of cystic echinococcosis (CE). This study was performed to examine and identify the molecular hydatid cyst in the dissected human specimens in paraffin tissue, and the dissected animal cyst was characterized using the DNA polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of internal transcribed spacer 1 (ITS1).

Materials And Methods: To determine the molecular properties of , 20 hydatid cyst samples (including 6 sheep samples, 9 camel samples, and 10 human paraffin samples) were collected from Zahedan and Zabol cities. After DNA extraction, molecular PCR was performed, and RFLP was evaluated. In this study, the endonuclease cleavage enzyme was used.

Results: The patterns of DNA bands found in the isolates from human CE and animal bladder cysts were the same, as indicated by the results of ribosomal DNA-ITS1 amplification from . Two nested primer pairs were used. The rough size of the enhanced ITS1 piece was 444 and 391 base pairs (bp), individually. After cutting the PCR product with the enzyme, the patterns of the fragments revealed that the samples had two identical RFLP patterns. The aftereffects of this study showed that the parasite genotypes confined to sheep, camels, and people had hereditary changes.

Conclusion: The transcendent type of in the area is , which featured the meaning of the sheep/canine cycle in human transmission. Albeit the band profile in the camel is now and again like the sheep strain, RLFP can be recognized utilizing the PCR strategy, and two differentiating band profiles using the chemical were found in this review.

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