Occurrence and Genetic Characterization of Echinococcus Granulosus Sensu Lato from Domestic Animals in Central Iran

Overview

Journal BMC Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2022 Jan 8

PMID 34996460

Citations 5

Authors

Bahador Hajimohammadi

Abdolhossein Dalimi

Gilda Eslami

Salman Ahmadian

Sajad Zandi

Ahmad Baghbani

Saeedeh Sadat Hosseini

Vahideh Askari

Maryam Sheykhzadegan

Mehrnoosh Nabizadeh Ardekani

Mohammad Javad Boozhmehrani

Mohammad Javad Ranjbar

Hamed Ghoshouni

Mahmood Vakili

Affiliations

Soon will be listed here.

Abstract

Background: The species complex of Echinococcus granulosus sensu lato (s.l.) causes cystic echinococcosis distributed worldwide. There is no genotype information from hydatid cysts in the intermediate hosts in Central Iran. Therefore, in this study, we analyzed the hydatid cysts in livestock slaughtered in an abattoir in this region. Six hundred fifty-seven hydatid cysts were isolated from 97 animals, including sheep, cattle, camels, and goats slaughtered in Yazd abattoir from September 2018 to January 2020. The demographic data was collected as well as cyst location, fertility, and viability. Out of 657 samples, 164 samples were genotyped. Then, phylogenetic analysis was performed using MEGAX. Statistical analyses were done using SPSS version 16.0 by chi-square with a significant difference of less than 0.05.

Results: Out of 164 samples, the G1-G3 complex genotype had the most frequency in samples, with 135 cases recognized. The G6/G7 was observed in 19 isolates and G5 was reported in nine samples. One sample was detected as Taenia hydatigena.

Conclusions: This study showed that G1-G3 and G6/G7 genotypes were presented in all animals, but G5 was reported only in cattle, goats, and camels. It is the first molecular identification of cystic echinococcosis in Central Iran. Hence, reporting G5 in livestock in this area should be considered due to transmission to humans.

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References

Latif A, Tanveer A, Maqbool A, Siddiqi N, Kyaw-Tanner M, Traub R . Morphological and molecular characterisation of Echinococcus granulosus in livestock and humans in Punjab, Pakistan. Vet Parasitol. 2010; 170(1-2):44-9. DOI: 10.1016/j.vetpar.2010.02.003. View

Rinaldi L, Maurelli M, Capuano F, Perugini A, Veneziano V, Cringoli S . Molecular update on cystic echinococcosis in cattle and water buffaloes of southern Italy. Zoonoses Public Health. 2008; 55(2):119-23. DOI: 10.1111/j.1863-2378.2007.01101.x. View

Pour A, Hosseini S, Shayan P . The prevalence and fertility of hydatid cysts in buffaloes from Iran. J Helminthol. 2011; 86(3):373-7. DOI: 10.1017/S0022149X11000514. View

Kebede W, Hagos A, Girma Z, Lobago F . Echinococcosis/hydatidosis: its prevalence, economic and public health significance in Tigray region, North Ethiopia. Trop Anim Health Prod. 2008; 41(6):865-71. DOI: 10.1007/s11250-008-9264-9. View

Bosco A, Alves L, Cociancic P, Amadesi A, Pepe P, Morgoglione M . Epidemiology and spatial distribution of Echinococcus granulosus in sheep and goats slaughtered in a hyperendemic European Mediterranean area. Parasit Vectors. 2021; 14(1):421. PMC: 8380321. DOI: 10.1186/s13071-021-04934-9. View

Pednekar R, Gatne M, Thompson R, Traub R . Molecular and morphological characterisation of Echinococcus from food producing animals in India. Vet Parasitol. 2009; 165(1-2):58-65. DOI: 10.1016/j.vetpar.2009.06.021. View

Ebrahimipour M, Sadjjadi S, Darani H, Najjari M . Molecular Studies on Cystic Echinococcosis of Camel () and Report of in Iran. Iran J Parasitol. 2017; 12(3):323-331. PMC: 5623911. View

Grosso G, Gruttadauria S, Biondi A, Marventano S, Mistretta A . Worldwide epidemiology of liver hydatidosis including the Mediterranean area. World J Gastroenterol. 2012; 18(13):1425-37. PMC: 3319938. DOI: 10.3748/wjg.v18.i13.1425. View

Kamenetzky L, Gutierrez A, Canova S, Haag K, Guarnera E, Parra A . Several strains of Echinococcus granulosus infect livestock and humans in Argentina. Infect Genet Evol. 2003; 2(2):129-36. DOI: 10.1016/s1567-1348(02)00131-4. View

10.

Singh B, Sharma J, Tuli A, Sharma R, Bal M, Aulakh R . Prevalence and morphological characterisation of Echinococcus granulosus from north India. J Parasit Dis. 2014; 38(1):36-40. PMC: 3909587. DOI: 10.1007/s12639-012-0189-x. View

11.

Umhang G, Richomme C, Boucher J, Hormaz V, Boue F . Prevalence survey and first molecular characterization of Echinococcus granulosus in France. Parasitol Res. 2012; 112(4):1809-12. PMC: 3597331. DOI: 10.1007/s00436-012-3245-7. View

12.

Varcasia A, Dessi G, Lattanzio S, Marongiu D, Cuccuru C, Carta S . Cystic echinococcosis in the endemic island of Sardinia (Italy): has something changed?. Parasitol Res. 2020; 119(7):2207-2215. DOI: 10.1007/s00436-020-06717-0. View

13.

Kinkar L, Laurimae T, Acosta-Jamett G, Andresiuk V, Balkaya I, Casulli A . Global phylogeography and genetic diversity of the zoonotic tapeworm Echinococcus granulosus sensu stricto genotype G1. Int J Parasitol. 2018; 48(9-10):729-742. DOI: 10.1016/j.ijpara.2018.03.006. View

14.

Rajabloo M, Hosseini S, Jalousian F . Morphological and molecular characterisation of Echinococcus granulosus from goat isolates in Iran. Acta Trop. 2012; 123(2):67-71. DOI: 10.1016/j.actatropica.2012.03.006. View

15.

Khademvatan S, Majidiani H, Foroutan M, Hazrati Tappeh K, Aryamand S, Khalkhali H . Echinococcus granulosus genotypes in Iran: a systematic review. J Helminthol. 2018; 93(2):131-138. DOI: 10.1017/S0022149X18000275. View

16.

Capuano F, Rinaldi L, Maurelli M, Perugini A, Veneziano V, Garippa G . Cystic echinococcosis in water buffaloes: epidemiological survey and molecular evidence of ovine (G1) and buffalo (G3) strains. Vet Parasitol. 2006; 137(3-4):262-8. DOI: 10.1016/j.vetpar.2006.01.016. View

17.

Boubaker G, Macchiaroli N, Prada L, Cucher M, Rosenzvit M, Ziadinov I . A multiplex PCR for the simultaneous detection and genotyping of the Echinococcus granulosus complex. PLoS Negl Trop Dis. 2013; 7(1):e2017. PMC: 3547860. DOI: 10.1371/journal.pntd.0002017. View

18.

Macin S, Orsten S, Samadzade R, Colak B, Cebeci H, Findik D . Human and animal cystic echinococcosis in Konya, Turkey: molecular identification and the first report of E. equinus from human host in Turkey. Parasitol Res. 2021; 120(2):563-568. PMC: 7813620. DOI: 10.1007/s00436-021-07050-w. View

19.

Notredame C, Higgins D, Heringa J . T-Coffee: A novel method for fast and accurate multiple sequence alignment. J Mol Biol. 2000; 302(1):205-17. DOI: 10.1006/jmbi.2000.4042. View

20.

Gessese A . Review on Epidemiology and Public Health Significance of Hydatidosis. Vet Med Int. 2020; 2020:8859116. PMC: 7735834. DOI: 10.1155/2020/8859116. View