Rapid, Low-input, Low-bias Construction of Shotgun Fragment Libraries by High-density in Vitro Transposition

Overview

Journal Genome Biol

Specialties Biology
Genetics

Date 2010 Dec 15

PMID 21143862

Citations 314

Authors

Andrew Adey

Hilary G Morrison

Asan

Xu Xun

Jacob O Kitzman

Emily H Turner

Bethany Stackhouse

Alexandra P MacKenzie

Nicholas C Caruccio

Xiuqing Zhang

Jay Shendure

Affiliations

Soon will be listed here.

Abstract

We characterize and extend a highly efficient method for constructing shotgun fragment libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation simultaneously. We apply this method to sequencing a human genome and find that coverage biases are comparable to those of conventional protocols. We also extend its capabilities by developing protocols for sub-nanogram library construction, exome capture from 50 ng of input DNA, PCR-free and colony PCR library construction, and 96-plex sample indexing.

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