A Large Genome Center's Improvements to the Illumina Sequencing System

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2008 Nov 27

PMID 19034268

Citations 406

Authors

Michael A Quail

Iwanka Kozarewa

Frances Smith

Aylwyn Scally

Philip J Stephens

Richard Durbin

Harold Swerdlow

Daniel J Turner

Affiliations

Soon will be listed here.

Abstract

The Wellcome Trust Sanger Institute is one of the world's largest genome centers, and a substantial amount of our sequencing is performed with 'next-generation' massively parallel sequencing technologies: in June 2008 the quantity of purity-filtered sequence data generated by our Genome Analyzer (Illumina) platforms reached 1 terabase, and our average weekly Illumina production output is currently 64 gigabases. Here we describe a set of improvements we have made to the standard Illumina protocols to make the library preparation more reliable in a high-throughput environment, to reduce bias, tighten insert size distribution and reliably obtain high yields of data.

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