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Thomas R M Barends

Explore the profile of Thomas R M Barends including associated specialties, affiliations and a list of published articles. Areas
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Articles 84
Citations 3205
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Recent Articles
1.
Akram M, Akram M, Hauser D, Hauser D, Dietl A, Dietl A, et al.
J Biol Chem . 2024 Dec; 301(2):108082. PMID: 39675707
The electrochemical potentials of redox-active proteins need to be tuned accurately to the correct values for proper biological function. Here, we describe a diheme cytochrome c with high heme redox...
2.
Doak R, Shoeman R, Gorel A, Nizinski S, Barends T, Schlichting I
J Appl Crystallogr . 2024 Dec; 57(Pt 6):1725-1732. PMID: 39628875
Serial crystallography (SX) efficiently distributes over many crystals the radiation dose absorbed during diffraction data acquisition, enabling structure determination of samples at ambient temperature. SX relies on the rapid and...
3.
Barends T, Gorel A, Bhattacharyya S, Schiro G, Bacellar C, Cirelli C, et al.
Nature . 2024 Feb; 626(8000):905-911. PMID: 38355794
High-intensity femtosecond pulses from an X-ray free-electron laser enable pump-probe experiments for the investigation of electronic and nuclear changes during light-induced reactions. On timescales ranging from femtoseconds to milliseconds and...
4.
Doak R, Shoeman R, Gorel A, Barends T, Marekha B, Haacke S, et al.
J Appl Crystallogr . 2023 Jun; 56(Pt 3):903-907. PMID: 37284264
A 'catcher' based on a revolving cylindrical collector is described. The simple and inexpensive device reduces free-jet instabilities inherent to high-viscosity extrusion injection, facilitating delivery of microcrystals for serial diffraction...
5.
Dietl A, Wellach K, Mahadevan P, Mertes N, Winter S, Kutsch T, et al.
J Biol Chem . 2023 Mar; 299(5):104602. PMID: 36907440
The genomes of anaerobic ammonium-oxidizing (anammox) bacteria contain a gene cluster comprising genes of unusual fatty acid biosynthesis enzymes that were suggested to be involved in the synthesis of the...
6.
Barends T, Stauch B, Cherezov V, Schlichting I
Nat Rev Methods Primers . 2023 Jan; 2. PMID: 36643971
With the advent of X-ray Free Electron Lasers (XFELs), new, high-throughput serial crystallography techniques for macromolecular structure determination have emerged. Serial femtosecond crystallography (SFX) and related methods provide possibilities beyond...
7.
Hadjidemetriou K, Coquelle N, Barends T, De Zitter E, Schlichting I, Colletier J, et al.
Acta Crystallogr D Struct Biol . 2022 Sep; 78(Pt 9):1131-1142. PMID: 36048153
Upon absorption of a blue-light photon, fatty-acid photodecarboxylase catalyzes the decarboxylation of free fatty acids to form hydrocarbons (for example alkanes or alkenes). The major components of the catalytic mechanism...
8.
Adam V, Hadjidemetriou K, Jensen N, Shoeman R, Woodhouse J, Aquila A, et al.
Chemphyschem . 2022 Aug; 23(19):e202200192. PMID: 35959919
Reversibly photoswitchable fluorescent proteins are essential markers for advanced biological imaging, and optimization of their photophysical properties underlies improved performance and novel applications. Here we establish a link between photoswitching...
9.
Tetreau G, Sawaya M, De Zitter E, Andreeva E, Banneville A, Schibrowsky N, et al.
Nat Commun . 2022 Jul; 13(1):4376. PMID: 35902572
Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis and jegathesan, respectively. The toxins naturally crystallize within the host; however, the crystals are...
10.
Grunbein M, Nass Kovacs G, Kloos M, Gorel A, Doak R, Shoeman R, et al.
Methods Mol Biol . 2022 Jul; 2501:147-168. PMID: 35857227
Crystal structures have provided detailed insight in the architecture of rhodopsin photoreceptors. Of particular interest are the protein-chromophore interactions that govern the light-induced retinal isomerization and ultimately induce the large...