Richard G Discipio
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Explore the profile of Richard G Discipio including associated specialties, affiliations and a list of published articles.
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Articles
18
Citations
503
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Recent Articles
1.
Discipio R, Schraufstatter I
J Immunol Methods
. 2017 Aug;
450:50-57.
PMID: 28757372
Two novel magnetic agarose bead based assays have been developed to measure complement component C5 interaction with C3b and the Factor I Modules (FIMs) of C7. One innovation was to...
2.
Discipio R, Liddington R, Schraufstatter I
Protein Expr Purif
. 2016 Jan;
121:118-24.
PMID: 26826315
A method is reported to purify Fibulin-1 from human plasma resulting in a 36% recovery. The steps involve removal of the cryoglobulin and the vitamin K dependent proteins followed by...
3.
Schraufstatter I, Khaldoyanidi S, Discipio R
World J Stem Cells
. 2015 Oct;
7(8):1090-108.
PMID: 26435769
The complement pathway is best known for its role in immune surveillance and inflammation. However, its ability of opsonizing and removing not only pathogens, but also necrotic and apoptotic cells,...
4.
Aleshin A, Discipio R, Stec B, Liddington R
J Biol Chem
. 2012 Apr;
287(23):19642-52.
PMID: 22500023
The complement membrane attack complex (MAC) forms transmembrane pores in pathogen membranes. The first step in MAC assembly is cleavage of C5 to generate metastable C5b, which forms a stable...
5.
Aleshin A, Schraufstatter I, Stec B, Bankston L, Liddington R, Discipio R
J Biol Chem
. 2012 Jan;
287(13):10210-10222.
PMID: 22267737
The complement membrane attack complex (MAC) is formed by the sequential assembly of C5b with four homologous proteins as follows: one copy each of C6, C7, and C8 and 12-14...
6.
Schraufstatter I, Zhao M, Khaldoyanidi S, Discipio R
Immunology
. 2011 Nov;
135(4):287-98.
PMID: 22117697
The observation that human monocytes cultured in the presence of the chemokine CCL18 showed increased survival, led us to profile cytokine expression in CCL18-stimulated versus control cultures. CCL18 caused significantly...
7.
Schraufstatter I, Discipio R, Khaldoyanidi S
Front Biosci (Landmark Ed)
. 2011 May;
16(6):2271-88.
PMID: 21622176
Mesenchymal stem cells (MSC) are multipotent stem cells that hold promise for an expanding list of therapeutic uses, not only due to their ability to differentiate into all connective tissues...
8.
Discipio R, Khaldoyanidi S, Schraufstatter I
Protein Expr Purif
. 2011 May;
79(1):72-80.
PMID: 21550406
An expression method has been developed to produce soluble cationic polypeptides in Escherichia coli while avoiding inclusion body deposition. For this technique the recombinant product is linked through a thrombin...
9.
Schraufstatter I, Discipio R, Khaldoyanidi S
J Stem Cells
. 2010 Aug;
4(4):203-15.
PMID: 20720594
The efficient migration of mesenchymal stem cells (MSCs) to diseased tissues is required for the fulfillment of their regenerative potential. Recruitment of circulating cells into the damaged tissues is regulated...
10.
Schraufstatter I, Discipio R, Zhao M, Khaldoyanidi S
J Immunol
. 2009 Mar;
182(6):3827-36.
PMID: 19265162
Mesenchymal stem cells (MSCs) have a great potential for tissue repair, especially if they can be delivered efficiently to sites of tissue injury. Since complement activation occurs whenever there is...