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Protein Expression and Purification

Protein Expression and Purification is a peer-reviewed scientific journal that focuses on the methods and techniques used to express and purify proteins for various applications. It covers topics such as recombinant protein production, protein engineering, protein purification strategies, and characterization of protein expression systems. The journal serves as a valuable resource for researchers and scientists in the field of protein biochemistry and biotechnology.

Details
Abbr. Protein Expr Purif
Start 1990
End Continuing
Frequency Monthly, 2003-
p-ISSN 1046-5928
e-ISSN 1096-0279
Country United States
Language English
Specialty Molecular Biology
Metrics
h-index / Ranks: 3337 96
SJR / Ranks: 10641 383
CiteScore / Ranks: 7049 3.80
JIF / Ranks: 5932 1.6
Recent Articles
1.
Ding Y, Jia L, Geng Q, Liu Y, Guo S, Zhao S, et al.
Protein Expr Purif . 2025 Mar; :106702. PMID: 40089126
The transferrin receptor (TfR1) mediates the cellular uptake of iron and other molecules, playing a vital role in hematology and tumor growth. Nanobodies(NBs) targeting TfR1 offer promising therapeutic potential due...
2.
Sudaraka Tennakoon M, Lee K, Shin H
Protein Expr Purif . 2025 Mar; :106700. PMID: 40086537
Ferritin is a versatile biomolecule used in various medical applications such as drug delivery, vaccines, biological imaging, and diagnostics. The purity and concentration of the ferritin nanoparticles are crucial for...
3.
Chen Q, Zhang Y, Zhang Y, Han X, Zhang L, Meng H, et al.
Protein Expr Purif . 2025 Mar; 230:106698. PMID: 40054512
The triple helix and D-period are distinctive features of native collagen, crucial for its physicochemical properties and bioactivities. However, developing recombinant humanized collagen with D-period features remains elusive. Here, we...
4.
Wang H, Zhang X, Yin C, Liu L, Chen L, Tang H, et al.
Protein Expr Purif . 2025 Mar; 230:106697. PMID: 40049376
Tumor necrosis factor receptor superfamily member 4 (OX40) is essential for the activation and maintenance of T cell immune function because of its recognition and regulation in signal transduction, therefore...
5.
Arakawa T, Ejima D, Tomioka Y, Sakuma C, Akuta T
Protein Expr Purif . 2025 Feb; 230:106690. PMID: 39978707
We review here several niche downstream purification processes that are not covered by other articles in this special issue. The first is the use of activated carbon to capture contaminants...
6.
Li Z, Lin T, Sun Y, Wang X, Yang Y, Gan L, et al.
Protein Expr Purif . 2025 Feb; 230:106689. PMID: 39971172
Glutaredoxin (Grx) is a small molecular protein widely found in both prokaryotes and eukaryotes, serving various biological functions, including participation in redox reactions and exerting anti-apoptotic effects[1]. To evaluate the...
7.
Zamani E, Zargan J, Honari H, Keshavarz Alikhani H
Protein Expr Purif . 2025 Feb; 229:106688. PMID: 39956441
Background And Objectives: Polyclonal antibodies are essential for identifying compounds like peptides. However, many peptides exhibit low immunogenicity, resulting in reduced antibody yields. To address this, fusing peptides with immunogenic...
8.
Shilling P, Pontes-Braz L, Mitchell L, Howell L, Veneer P, Jayashree S, et al.
Protein Expr Purif . 2025 Feb; 229:106683. PMID: 39922437
Rational design of fundamentally new advanced materials would be facilitated by availability of polymers with controlled monomer sequence. Recombinant proteins offer polymers with controlled monomer sequence but are underrepresented in...
9.
Hu J, Feng W, Wen J, Zhou S, Sun Z, Zhao S, et al.
Protein Expr Purif . 2025 Feb; 229:106681. PMID: 39921134
Aims: IL-12B, a subunit of the IL-23 family of cytokines, plays a crucial role in various diseases such as viral infections, autoimmune disorders, and tumors. This study aimed to identify...
10.
Fei S, Hu W, Shu J, Zhao R, Zhao J, Jiang M, et al.
Protein Expr Purif . 2025 Feb; 229:106687. PMID: 39914789
Xylitol dehydrogenase (XDH; EC 1.1.1.9), encoded by the XYL2 gene, is a key enzyme in the fungal xylose metabolic pathway. In this work, a putative XDH from the plant endophytic...