Richard A J Darby
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Explore the profile of Richard A J Darby including associated specialties, affiliations and a list of published articles.
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Articles
14
Citations
157
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Recent Articles
1.
Cartwright S, Darby R, Sarkar D, Bonander N, Gross S, Ashe M, et al.
Microb Cell Fact
. 2017 Mar;
16(1):41.
PMID: 28279185
Background: We previously selected four strains of Saccharomyces cerevisiae for their ability to produce the aquaporin Fps1 in sufficient yield for further study. Yields from the yeast strains spt3Δ, srb5Δ,...
2.
Darby R, Unsworth A, Knapp S, Kerr I, Callaghan R
Cancer Chemother Pharmacol
. 2015 Sep;
76(4):853-64.
PMID: 26351135
Purpose: Multidrug efflux pumps such as ABCG2 confer drug resistance to a number of cancer types, leading to poor prognosis and outcome. To date, the strategy of directly inhibiting multidrug...
3.
Jamshad M, Darby R
Methods Mol Biol
. 2012 Mar;
866:237-46.
PMID: 22454128
Yeast is a proven host for the production of recombinant proteins, which may be incorporated in cellular membranes or localized in subcellular compartments. In order to gain access to these...
4.
Bawa Z, Darby R
Methods Mol Biol
. 2012 Mar;
866:181-90.
PMID: 22454123
A common method for inducing the production of recombinant proteins in Pichia pastoris is through the use of methanol. However, the by-products of methanol metabolism are toxic to yeast cells...
5.
Jamshad M, Darby R
Methods Mol Biol
. 2012 Mar;
866:57-63.
PMID: 22454114
There are several ways to introduce non-native DNA into yeast cells, including chemical transformation and electroporation. Methods for both of these procedures are outlined in this chapter. Both methods permit...
6.
Darby R, Cartwright S, Dilworth M, Bill R
Methods Mol Biol
. 2012 Mar;
866:11-23.
PMID: 22454110
Having decided on yeast as a production host, the choice of species is often the first question any researcher new to the field will ask. With over 500 known species...
7.
Bawa Z, Bland C, Bonander N, Bora N, Cartwright S, Clare M, et al.
Biochem Soc Trans
. 2011 May;
39(3):719-23.
PMID: 21599640
Membrane proteins are drug targets for a wide range of diseases. Having access to appropriate samples for further research underpins the pharmaceutical industry's strategy for developing new drugs. This is...
8.
Darby R, Callaghan R, McMahon R
Curr Drug Metab
. 2011 Mar;
12(8):722-31.
PMID: 21434857
The multidrug resistant phenotype of cancer cells can often result from the over-production of a number of ATP binding cassette (ABC) transporters, including P-glycoprotein (P-gp). These multidrug efflux transporters expel...
9.
Ferndahl C, Bonander N, Logez C, Wagner R, Gustafsson L, Larsson C, et al.
Microb Cell Fact
. 2010 Jun;
9:47.
PMID: 20565740
Background: Recombinant protein production is universally employed as a solution to obtain the milligram to gram quantities of a given protein required for applications as diverse as structural genomics and...
10.
Darby R, Forde G, Slater N, Hine A
Biotechnol Bioeng
. 2007 May;
98(5):1103-8.
PMID: 17497740
We have shown previously that a sequence-specific DNA-binding protein based on the Lac repressor protein can isolate pre-purified DNA efficiently from simple buffer solution but our attempts to purify plasmids...