Douglas A Bernstein
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Explore the profile of Douglas A Bernstein including associated specialties, affiliations and a list of published articles.
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28
Citations
1523
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Recent Articles
1.
Walther R, Hrabak M, Bernstein D
J Microbiol Biol Educ
. 2024 Jul;
25(2):e0006124.
PMID: 38975770
Molecular biology, broadly defined as the investigation of complex biomolecules in the laboratory, is a rapidly advancing field and as such the technologies available to investigators are constantly evolving. This...
2.
Doss E, Moore J, Harman B, Doud E, Rubenstein E, Bernstein D
PeerJ
. 2023 Aug;
11:e15897.
PMID: 37645016
Background: is the most prevalent human fungal pathogen. In immunocompromised individuals, can cause serious systemic disease, and patients infected with drug-resistant isolates have few treatment options. The ubiquitin-proteasome system has...
3.
Evans B, Bernstein D
Methods Mol Biol
. 2022 Aug;
2542:3-12.
PMID: 36008653
Cas9-mediated genome editing is one tool investigators can use to study fungal pathogens. Such methodologies allow the investigator to examine how fungal cells differ from human cells and thus potentially...
4.
Evans B, Pickerill E, Bernstein D
J Microbiol Biol Educ
. 2021 Oct;
22(2).
PMID: 34594464
Course-based undergraduate research experiences (CURE) offer the chance for students to experience authentic research investigation in a classroom setting. Such hands-on experiences afford unique opportunities work on a semi-independent research...
5.
Pickerill E, Bernstein D
Methods Enzymol
. 2021 Sep;
658:435-452.
PMID: 34517958
Though over 100 distinct RNA modifications have been identified, the roles for many of these modifications in vivo remain unknown. Genome editing is one tool investigators are using to better...
6.
Evans B, Bernstein D
mSphere
. 2021 May;
6(3).
PMID: 34011687
is a human fungal pathogen capable of causing life-threatening infections. The ability to edit the genome using CRISPR/Cas9 is an important tool investigators can leverage in their search for novel...
7.
Vyas V, Bernstein D
J Microbiol Biol Educ
. 2020 Jan;
20(3).
PMID: 31890080
Central dogma, transformation, and genome editing are key biological concepts for which junior scientists must gain experience during training. Here we present an exercise that introduces these concepts in a...
8.
Pickerill E, Kurtz R, Tharp A, Guerrero Sanz P, Begum M, Bernstein D
Yeast
. 2019 Aug;
36(11):669-677.
PMID: 31364194
RNA can be modified in over 100 distinct ways, and these modifications are critical for function. Pseudouridine synthases catalyse pseudouridylation, one of the most prevalent RNA modifications. Pseudouridine synthase 7...
9.
Evans B, Pickerill E, Vyas V, Bernstein D
J Vis Exp
. 2018 Dec;
(141).
PMID: 30507925
This method describes the efficient CRISPR mediated genome editing of the diploid human fungal pathogen Candida albicans. CRISPR-mediated genome editing in C. albicans requires Cas9, guide RNA, and repair template....
10.
Chambers A, Richardson A, Read D, Waller T, Bernstein D, Smaldino P
J Vis Exp
. 2018 Oct;
(140).
PMID: 30346392
N-isopentenyladenosine RNA modifications are functionally diverse and highly conserved among prokaryotes and eukaryotes. One of the most highly conserved N-isopentenyladenosine modifications occurs at the A37 position in a subset of...