Fluorescence Tomographic Imaging in Turbid Media Using Early-arriving Photons and Laplace Transforms

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1997 Aug 5

PMID 9238055

Citations 30

Authors

J Wu

L Perelman

R R Dasari

M S Feld

Affiliations

Soon will be listed here.

Abstract

We present a multichannel tomographic technique to detect fluorescent objects embedded in thick (6.4 cm) tissue-like turbid media using early-arriving photons. The experiments use picosecond laser pulses and a streak camera with single photon counting capability to provide short time resolution and high signal-to-noise ratio. The tomographic algorithm is based on the Laplace transform of an analytical diffusion approximation of the photon migration process and provides excellent agreement between the actual positions of the fluorescent objects and the experimental estimates. Submillimeter localization accuracy and 4- to 5-mm resolution are demonstrated. Moreover, objects can be accurately localized when fluorescence background is present. The results show the feasibility of using early-arriving photons to image fluorescent objects embedded in a turbid medium and its potential in clinical applications such as breast tumor detection.

Citing Articles

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Review of structured light in diffuse optical imaging.

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Laplace-domain diffuse optical measurement.

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Single-shot real-time video recording of a photonic Mach cone induced by a scattered light pulse.

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References

Fitzmaurice M, Bordagaray J, Engelmann G, Richards-Kortum R, Kolubayev T, Feld M . Argon ion laser-excited autofluorescence in normal and atherosclerotic aorta and coronary arteries: morphologic studies. Am Heart J. 1989; 118(5 Pt 1):1028-38. DOI: 10.1016/0002-8703(89)90239-1. View

Wu J, Wang Y, Perelman L, Itzkan I, Dasari R, Feld M . Time-resolved multichannel imaging of fluorescent objects embedded in turbid media. Opt Lett. 2009; 20(5):489-91. DOI: 10.1364/ol.20.000489. View

Perelman , Wu , Wang , Itzkan I , Dasari , FELD . Time-dependent photon migration using path integrals. Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics. 1995; 51(6):6134-6141. DOI: 10.1103/physreve.51.6134. View

Benaron D, Stevenson D . Optical time-of-flight and absorbance imaging of biologic media. Science. 1993; 259(5100):1463-6. DOI: 10.1126/science.8451643. View

Hee M, Izatt J, Jacobson J, Fujimoto J, Swanson E . Femtosecond transillumination optical coherence tomography. Opt Lett. 2009; 18(12):950-2. DOI: 10.1364/ol.18.000950. View

Mitic G, Kolzer J, Otto J, Plies E, Solkner G, Zinth W . Time-gated transillumination of biological tissues and tissuelike phantoms. Appl Opt. 2010; 33(28):6699-710. DOI: 10.1364/AO.33.006699. View

Nishiwaki Y, Nakamura S, Sakaguchi S . New method of photosensitizer accumulation for photodynamic therapy in an experimental liver tumor. Lasers Surg Med. 1989; 9(3):254-63. DOI: 10.1002/lsm.1900090308. View

Wang L, Ho P, Alfano R . Double-stage picosecond Kerr gate for ballistic time-gated optical imaging in turbid media. Appl Opt. 2010; 32(4):535-40. DOI: 10.1364/AO.32.000535. View

Berg R, Jarlman O, Svanberg S . Medical transillumination imaging using short-pulse diode lasers. Appl Opt. 2010; 32(4):574-9. DOI: 10.1364/AO.32.000574. View

10.

Wu J . Convolution picture of the boundary conditions in photon migration and its implications in time-resolved optical imaging of biological tissues. J Opt Soc Am A Opt Image Sci Vis. 1997; 14(1):280-7. DOI: 10.1364/josaa.14.000280. View

11.

BOAS , OLeary , Chance , Yodh . Scattering and wavelength transduction of diffuse photon density waves. Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics. 1993; 47(5):R2999-R3002. DOI: 10.1103/physreve.47.r2999. View

12.

Patterson M, Chance B, Wilson B . Time resolved reflectance and transmittance for the non-invasive measurement of tissue optical properties. Appl Opt. 2010; 28(12):2331-6. DOI: 10.1364/AO.28.002331. View

13.

OLeary , BOAS , Chance , Yodh . Refraction of diffuse photon density waves. Phys Rev Lett. 1992; 69(18):2658-2661. DOI: 10.1103/PhysRevLett.69.2658. View

14.

Wu J, Wang Y, Perelman L, Itzkan I, Dasari R, Feld M . Three-dimensional imaging of objects embedded in turbid media with fluorescence and Raman spectroscopy. Appl Opt. 2010; 34(18):3425-30. DOI: 10.1364/AO.34.003425. View