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Monte Carlo Based Method for Fluorescence Tomographic Imaging with Lifetime Multiplexing Using Time Gates

Overview
Specialty Radiology
Date 2011 Apr 13
PMID 21483610
Citations 42
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Abstract

Time-resolved fluorescence optical tomography allows 3-dimensional localization of multiple fluorophores based on lifetime contrast while providing a unique data set for improved resolution. However, to employ the full fluorescence time measurements, a light propagation model that accurately simulates weakly diffused and multiple scattered photons is required. In this article, we derive a computationally efficient Monte Carlo based method to compute time-gated fluorescence Jacobians for the simultaneous imaging of two fluorophores with lifetime contrast. The Monte Carlo based formulation is validated on a synthetic murine model simulating the uptake in the kidneys of two distinct fluorophores with lifetime contrast. Experimentally, the method is validated using capillaries filled with 2.5nmol of ICG and IRDye™800CW respectively embedded in a diffuse media mimicking the average optical properties of mice. Combining multiple time gates in one inverse problem allows the simultaneous reconstruction of multiple fluorophores with increased resolution and minimal crosstalk using the proposed formulation.

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References
1.
Wu J, Perelman L, Dasari R, Feld M . Fluorescence tomographic imaging in turbid media using early-arriving photons and Laplace transforms. Proc Natl Acad Sci U S A. 1997; 94(16):8783-8. PMC: 23129. DOI: 10.1073/pnas.94.16.8783. View

2.
Venugopal V, Chen J, Lesage F, Intes X . Full-field time-resolved fluorescence tomography of small animals. Opt Lett. 2010; 35(19):3189-91. DOI: 10.1364/OL.35.003189. View

3.
Cheng X, Boas D . Systematic diffuse optical image errors resulting from uncertainty in the background optical properties. Opt Express. 2009; 4(8):299-307. DOI: 10.1364/oe.4.000299. View

4.
Suhling K, French P, Phillips D . Time-resolved fluorescence microscopy. Photochem Photobiol Sci. 2004; 4(1):13-22. DOI: 10.1039/b412924p. View

5.
Niedre M, de Kleine R, Aikawa E, Kirsch D, Weissleder R, Ntziachristos V . Early photon tomography allows fluorescence detection of lung carcinomas and disease progression in mice in vivo. Proc Natl Acad Sci U S A. 2008; 105(49):19126-31. PMC: 2614726. DOI: 10.1073/pnas.0804798105. View