Sequence and Characterization of a Novel Chromosomal Aminoglycoside Phosphotransferase Gene, Aph (3')-IIb, in Pseudomonas Aeruginosa

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 1996 May 1

PMID 8723476

Citations 24

Authors

H Hachler

P Santanam

F H Kayser

Affiliations

Soon will be listed here.

Abstract

A novel, probably chromosomally encoded, aminoglycoside phosphotransferase gene was cloned on a 2,996-bp PstI fragment from Pseudomonas aeruginosa and designated aph (3')-IIb. It coded for a protein of 268 amino acids that showed 51.7% amino acid identity with APH (3')-II [APH(3') is aminoglycoside-3' phosphotransferase] from Tn5. Two other open reading frames on the cloned fragment showed homology to a signal-transducing system in P. aeruginosa.

Citing Articles

Phenotypic and resistome analysis of antibiotic and heavy metal resistance in the Antarctic bacterium Pseudomonas sp. AU10.

Garcia-Lavina C, Morel M, Garcia-Gabarrot G, Castro-Sowinski S Braz J Microbiol. 2023; 54(4):2903-2913.

PMID: 37783937 PMC: 10689667. DOI: 10.1007/s42770-023-01135-7.

Exploring Oceans for Curative Compounds: Potential New Antimicrobial and Anti-Virulence Molecules against .

Coppola D, Buonocore C, Palisse M, Tedesco P, de Pascale D Mar Drugs. 2023; 21(1).

PMID: 36662182 PMC: 9865402. DOI: 10.3390/md21010009.

Pseudomonas aeruginosa: pathogenesis, virulence factors, antibiotic resistance, interaction with host, technology advances and emerging therapeutics.

Qin S, Xiao W, Zhou C, Pu Q, Deng X, Lan L Signal Transduct Target Ther. 2022; 7(1):199.

PMID: 35752612 PMC: 9233671. DOI: 10.1038/s41392-022-01056-1.

Metagenomics Reveals the Diversity and Taxonomy of Carbohydrate-Active Enzymes and Antibiotic Resistance Genes in Suancai Bacterial Communities.

Song Q, Wang B, Han Y, Zhou Z Genes (Basel). 2022; 13(5).

PMID: 35627157 PMC: 9141641. DOI: 10.3390/genes13050773.

A glimpse of antimicrobial resistance gene diversity in kefir and yoghurt.

Toth A, Csabai I, Maroti G, Jerzsele A, Dubecz A, Patai A Sci Rep. 2021; 10(1):22458.

PMID: 33384459 PMC: 7775456. DOI: 10.1038/s41598-020-80444-5.

References

Shaw K, Munayyer H, Rather P, Hare R, Miller G . Nucleotide sequence analysis and DNA hybridization studies of the ant(4')-IIa gene from Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1993; 37(4):708-14. PMC: 187739. DOI: 10.1128/AAC.37.4.708. View

Matsuhashi Y, Yagisawa M, Kondo S, Takeuchi T, Umezawa H . Aminoglycoside 3'-phosphotransferases I and II in Pseudomonas aeruginosa. J Antibiot (Tokyo). 1975; 28(6):442-7. DOI: 10.7164/antibiotics.28.442. View

Duval J, Soussy C . [Development and current status of Pseudomonas aeruginosa sensitivity to antibiotics]. Presse Med. 1984; 13(13):763-7. View

Young S, Tenover F, Gootz T, Gordon K, PLORDE J . Development of two DNA probes for differentiating the structural genes of subclasses I and II of the aminoglycoside-modifying enzyme 3'-aminoglycoside phosphotransferase. Antimicrob Agents Chemother. 1985; 27(5):739-44. PMC: 180144. DOI: 10.1128/AAC.27.5.739. View

Brenner S . Phosphotransferase sequence homology. Nature. 1987; 329(6134):21. DOI: 10.1038/329021a0. View

MARTIN P, Jullien E, Courvalin P . Nucleotide sequence of Acinetobacter baumannii aphA-6 gene: evolutionary and functional implications of sequence homologies with nucleotide-binding proteins, kinases and other aminoglycoside-modifying enzymes. Mol Microbiol. 1988; 2(5):615-25. DOI: 10.1111/j.1365-2958.1988.tb00070.x. View

Hobbs M, Collie E, Free P, Livingston S, Mattick J . PilS and PilR, a two-component transcriptional regulatory system controlling expression of type 4 fimbriae in Pseudomonas aeruginosa. Mol Microbiol. 1993; 7(5):669-82. DOI: 10.1111/j.1365-2958.1993.tb01158.x. View

Yenofsky R, Fine M, Pellow J . A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antibiotic selection pressure. Proc Natl Acad Sci U S A. 1990; 87(9):3435-9. PMC: 53915. DOI: 10.1073/pnas.87.9.3435. View

Jacoby G, Blaser M, Santanam P, Hachler H, Kayser F, Hare R . Appearance of amikacin and tobramycin resistance due to 4'-aminoglycoside nucleotidyltransferase [ANT(4')-II] in gram-negative pathogens. Antimicrob Agents Chemother. 1990; 34(12):2381-6. PMC: 172065. DOI: 10.1128/AAC.34.12.2381. View

10.

Blazquez J, Davies J, Moreno F . Mutations in the aphA-2 gene of transposon Tn5 mapping within the regions highly conserved in aminoglycoside-phosphotransferases strongly reduce aminoglycoside resistance. Mol Microbiol. 1991; 5(6):1511-8. DOI: 10.1111/j.1365-2958.1991.tb00798.x. View

11.

Kocabiyik S, Perlin M . Altered substrate specificity by substitutions at Tyr218 in bacterial aminoglycoside 3'-phosphotransferase-II. FEMS Microbiol Lett. 1992; 72(2):199-202. DOI: 10.1016/0378-1097(92)90529-w. View

12.

Delorme C, Ehrlich S, Renault P . Histidine biosynthesis genes in Lactococcus lactis subsp. lactis. J Bacteriol. 1992; 174(20):6571-9. PMC: 207627. DOI: 10.1128/jb.174.20.6571-6579.1992. View

13.

Shaw K, Rather P, Hare R, Miller G . Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes. Microbiol Rev. 1993; 57(1):138-63. PMC: 372903. DOI: 10.1128/mr.57.1.138-163.1993. View

14.

Tenover F, Gilbert T, OHara P . Nucleotide sequence of a novel kanamycin resistance gene, aphA-7, from Campylobacter jejuni and comparison to other kanamycin phosphotransferase genes. Plasmid. 1989; 22(1):52-8. DOI: 10.1016/0147-619x(89)90035-8. View