Overexpression and Initial Characterization of the Chromosomal Aminoglycoside 3'-O-phosphotransferase APH(3')-IIb from Pseudomonas Aeruginosa

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2006 Nov 8

PMID 17088479

Citations 6

Authors

Mariana Hainrichson

Orit Yaniv

Marina Cherniavsky

Igor Nudelman

Dalia Shallom-Shezifi

Sima Yaron

Timor Baasov

Affiliations

Soon will be listed here.

Abstract

The chromosomal gene aph(3')-IIb, encoding an aminoglycoside 3'-phosphotransferase in Pseudomonas aeruginosa, was cloned and overexpressed in Escherichia coli. The APH(3')-IIb enzyme was purified as a monomer in a two-step procedure and was shown to phosphorylate its substrates at the C-3'-OH position, with kcat/Km values of 0.4x10(4) to 36x10(4) M-1 s-1.

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