LTQ-XL Mass Spectrometry Proteome Analysis Expands the Pseudomonas Aeruginosa AmpR Regulon to Include Cyclic Di-GMP Phosphodiesterases and Phosphoproteins, and Identifies Novel Open Reading Frames

Overview

Journal J Proteomics

Publisher Elsevier

Specialty Biochemistry

Date 2013 Dec 3

PMID 24291602

Citations 9

Authors

Hansi Kumari

Senthil K Murugapiran

Deepak Balasubramanian

Lisa Schneper

Massimo Merighi

David Sarracino

Stephen Lory

Kalai Mathee

Affiliations

Soon will be listed here.

Abstract

Biological Significance: The AmpR proteome data not only confirmed the role of AmpR in virulence and resistance to multiple antibiotics, but also expanded the perimeter of AmpR regulon. The data presented here points to the role of AmpR in regulating cyclic di-GMP levels and phosphorylation of Ser, Thr and Tyr, adding another dimension to the regulatory functions of AmpR. We also identify some previously unannotated/misannotated ORFs in the P. aeruginosa genome, indicating the limitations of existing ORF analyses software. This study will contribute towards understanding complex genetic organization of P. aeruginosa. Whole genome proteomic picture of regulators at higher nodal positions in the regulatory network will not only help us link various virulence phenotypes but also design novel therapeutic strategies.

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