Elimination of L-A Double-stranded RNA Virus of Saccharomyces Cerevisiae by Expression of Gag and Gag-pol from an L-A CDNA Clone

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1993 May 1

PMID 8474174

Citations 21

Authors

R P VALLE

R B Wickner

Affiliations

Soon will be listed here.

Abstract

We report that expression of a nearly full-length cDNA clone of the L-A double-stranded RNA virus causes virus loss in a wild-type strain of Saccharomyces cerevisiae. We show that in this system exclusion of the L-A virus is independent of the presence of the packaging site or of cis sites for replication and transcription and completely dependent on expression of functional recombinant gag and gag-pol fusion protein. Thus, this exclusion is not explained in terms of overexpression of packaging signals. Mutation of the chromosomal SKI2 gene, known to repress the copy number of double-stranded RNA cytoplasmic replicons of S. cerevisiae, nearly eliminates the exclusion. We suggest that exclusion is due to competition by proteins expressed from the plasmid for a possibly limiting cellular factor. Our hypotheses on exclusion of L-A proteins may also apply to resistance to plant viruses produced by expression of viral replicases in transgenic plants.

Citing Articles

RNA viruses, M satellites, chromosomal killer genes, and killer/nonkiller phenotypes in the 100-genomes S. cerevisiae strains.

Vijayraghavan S, Kozmin S, Strope P, Skelly D, Magwene P, Dietrich F G3 (Bethesda). 2023; 13(10).

PMID: 37497616 PMC: 10542562. DOI: 10.1093/g3journal/jkad167.

Genome Organization of a New Double-Stranded RNA LA Helper Virus From Wine Killer Yeast as Compared With Its Counterparts.

Ramirez M, Velazquez R, Maqueda M, Martinez A Front Microbiol. 2020; 11:593846.

PMID: 33324373 PMC: 7721687. DOI: 10.3389/fmicb.2020.593846.

L-A-lus, a new variant of the L-A totivirus found in wine yeasts with Klus killer toxin-encoding Mlus double-stranded RNA: possible role of killer toxin-encoding satellite RNAs in the evolution of their helper viruses.

Rodriguez-Cousino N, Gomez P, Esteban R Appl Environ Microbiol. 2013; 79(15):4661-74.

PMID: 23728812 PMC: 3719527. DOI: 10.1128/AEM.00500-13.

Virus-host co-evolution under a modified nuclear genetic code.

Taylor D, Ballinger M, Bowman S, Bruenn J PeerJ. 2013; 1:e50.

PMID: 23638388 PMC: 3628385. DOI: 10.7717/peerj.50.

Compatibility with killer explains the rise of RNAi-deficient fungi.

Drinnenberg I, Fink G, Bartel D Science. 2011; 333(6049):1592.

PMID: 21921191 PMC: 3790311. DOI: 10.1126/science.1209575.

References

Welsh J, Leibowitz M, Wickner R . Virion DNA-independent RNA polymerase from Saccharomyces cerevisiae. Nucleic Acids Res. 1980; 8(11):2349-63. PMC: 324086. DOI: 10.1093/nar/8.11.2349. View

Somers J . Isolation of Suppressive Sensitive Mutants from Killer and Neutral Strains of SACCHAROMYCES CEREVISIAE. Genetics. 1973; 74(4):571-9. PMC: 1212973. DOI: 10.1093/genetics/74.4.571. View

Fujimura T, RIBAS J, Makhov A, Wickner R . Pol of gag-pol fusion protein required for encapsidation of viral RNA of yeast L-A virus. Nature. 1992; 359(6397):746-9. DOI: 10.1038/359746a0. View

Sherman F . Getting started with yeast. Methods Enzymol. 1991; 194:3-21. DOI: 10.1016/0076-6879(91)94004-v. View

Meier E, Harmison G, Schubert M . Homotypic and heterotypic exclusion of vesicular stomatitis virus replication by high levels of recombinant polymerase protein L. J Virol. 1987; 61(10):3133-42. PMC: 255890. DOI: 10.1128/JVI.61.10.3133-3142.1987. View

Wickner R . Plasmids controlled exclusion of the K2 killer double-stranded RNA plasmid of yeast. Cell. 1980; 21(1):217-26. DOI: 10.1016/0092-8674(80)90129-4. View

Thiele D, Hannig E, Leibowitz M . Genome structure and expression of a defective interfering mutant of the killer virus of yeast. Virology. 1984; 137(1):20-31. DOI: 10.1016/0042-6822(84)90004-7. View

Lee M, Pietras D, Nemeroff M, Corstanje B, Field L, Bruenn J . Conserved regions in defective interfering viral double-stranded RNAs from a yeast virus. J Virol. 1986; 58(2):402-7. PMC: 252925. DOI: 10.1128/JVI.58.2.402-407.1986. View

Icho T, Wickner R . The double-stranded RNA genome of yeast virus L-A encodes its own putative RNA polymerase by fusing two open reading frames. J Biol Chem. 1989; 264(12):6716-23. View

10.

Uemura H, Wickner R . Suppression of chromosomal mutations affecting M1 virus replication in Saccharomyces cerevisiae by a variant of a viral RNA segment (L-A) that encodes coat protein. Mol Cell Biol. 1988; 8(2):938-44. PMC: 363226. DOI: 10.1128/mcb.8.2.938-944.1988. View

11.

Tercero J, Riles L, Wickner R . Localized mutagenesis and evidence for post-transcriptional regulation of MAK3. A putative N-acetyltransferase required for double-stranded RNA virus propagation in Saccharomyces cerevisiae. J Biol Chem. 1992; 267(28):20270-6. View

12.

RIBAS J, Wickner R . RNA-dependent RNA polymerase consensus sequence of the L-A double-stranded RNA virus: definition of essential domains. Proc Natl Acad Sci U S A. 1992; 89(6):2185-9. PMC: 48621. DOI: 10.1073/pnas.89.6.2185. View

13.

Toh-E A, Sahashi Y . The PET18 locus of Saccharomyces cerevisiae: a complex locus containing multiple genes. Yeast. 1985; 1(2):159-71. DOI: 10.1002/yea.320010204. View

14.

Golemboski D, Lomonossoff G, Zaitlin M . Plants transformed with a tobacco mosaic virus nonstructural gene sequence are resistant to the virus. Proc Natl Acad Sci U S A. 1990; 87(16):6311-5. PMC: 54523. DOI: 10.1073/pnas.87.16.6311. View

15.

Inokuchi Y, Hirashima A . Interference with viral infection by defective RNA replicase. J Virol. 1987; 61(12):3946-9. PMC: 256014. DOI: 10.1128/JVI.61.12.3946-3949.1987. View

16.

Roux L, Simon A, Holland J . Effects of defective interfering viruses on virus replication and pathogenesis in vitro and in vivo. Adv Virus Res. 1991; 40:181-211. PMC: 7131706. DOI: 10.1016/s0065-3527(08)60279-1. View

17.

Klebe R, Harriss J, Sharp Z, DOUGLAS M . A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast. Gene. 1983; 25(2-3):333-41. DOI: 10.1016/0378-1119(83)90238-x. View

18.

Fujimura T, Esteban R, Esteban L, Wickner R . Portable encapsidation signal of the L-A double-stranded RNA virus of S. cerevisiae. Cell. 1990; 62(4):819-28. DOI: 10.1016/0092-8674(90)90125-x. View

19.

Vodkin M, Katterman F, Fink G . Yeast killer mutants with altered double-stranded ribonucleic acid. J Bacteriol. 1974; 117(2):681-6. PMC: 285560. DOI: 10.1128/jb.117.2.681-686.1974. View

20.

Huan B, Shen Y, Bruenn J . In vivo mapping of a sequence required for interference with the yeast killer virus. Proc Natl Acad Sci U S A. 1991; 88(4):1271-5. PMC: 50999. DOI: 10.1073/pnas.88.4.1271. View