Translational Maintenance of Frame: Mutants of Saccharomyces Cerevisiae with Altered -1 Ribosomal Frameshifting Efficiencies

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 1994 Jan 1

PMID 8138178

Citations 53

Authors

J D Dinman

R B Wickner

Affiliations

Soon will be listed here.

Abstract

A special site on the (+) strand of the L-A dsRNA virus induces about 2% of ribosomes translating the gag open reading frame to execute a -1 frameshift and thus produce the viral gag-pol fusion protein. Using constructs in which a -1 ribosomal frameshift at this site was necessary for expression of lacZ we isolated chromosomal mutants in which the efficiency of frameshifting was increased. These mutants comprise eight genes, named mof (maintenance of frame). The mof1-1, mof2-1, mof4-1, mof5-1 and mof6-1 strains cannot maintain M1 dsRNA at 30 degrees, but, paradoxically, do not lose L-A. The mof2-1, mof5-1 and mof6-1 strains are temperature sensitive for growth at 37 degrees, and all three show striking cell cycle phenotypes. The mof2-1 strains arrest with mother and daughter cells almost equal in size, mof5-1 arrests with multiple buds and mof6-1 arrests as single large unbudded cells. mof2-1 and mof5-1 strains are also Pet-. The mof mutations show differential effects on various frameshifting signals.

Citing Articles

The Deficiency of Hypusinated eIF5A Decreases the Putrescine/Spermidine Ratio and Inhibits +1 Programmed Ribosomal Frameshifting during the Translation of Ty1 Retrotransposon in .

Xiao Y, Wang R, Han X, Wang W, Liang A Int J Mol Sci. 2024; 25(3).

PMID: 38339043 PMC: 10855120. DOI: 10.3390/ijms25031766.

Reprogramming the genetic code: The emerging role of ribosomal frameshifting in regulating cellular gene expression.

Advani V, Dinman J Bioessays. 2015; 38(1):21-6.

PMID: 26661048 PMC: 4749135. DOI: 10.1002/bies.201500131.

Cell cycle control (and more) by programmed -1 ribosomal frameshifting: implications for disease and therapeutics.

Belew A, Dinman J Cell Cycle. 2015; 14(2):172-8.

PMID: 25584829 PMC: 4615106. DOI: 10.4161/15384101.2014.989123.

Ribosomes in the balance: structural equilibrium ensures translational fidelity and proper gene expression.

Musalgaonkar S, Moomau C, Dinman J Nucleic Acids Res. 2014; 42(21):13384-92.

PMID: 25389262 PMC: 4245932. DOI: 10.1093/nar/gku1020.

A cluster of methylations in the domain IV of 25S rRNA is required for ribosome stability.

Gigova A, Duggimpudi S, Pollex T, Schaefer M, Kos M RNA. 2014; 20(10):1632-44.

PMID: 25125595 PMC: 4174444. DOI: 10.1261/rna.043398.113.

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