A General Method for Polyethylene-glycol-induced Genetic Transformation of Bacteria and Yeast

Overview

Journal Gene

Specialty Molecular Biology

Date 1983 Nov 1

PMID 6363214

Citations 148

Authors

R J Klebe

J V Harriss

Z D Sharp

M G DOUGLAS

Affiliations

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Abstract

Polyethylene glycol (PEG) can induce genetic transformation in both bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) without cell wall removal. PEG-mediated transformation of E. coli is technically simple and yields transformants with an efficiency of 10(6)-10(7) transformants/microgram DNA. Detailed analysis of the parameters involved in PEG-mediated transformation of E. coli reveals basic differences between the PEG and standard CaCl2 methods for transformation of E. coli. PEG-mediated transformation of yeast is far simpler than existing protoplast methods and is comparable in efficiency. The new methods described here for PEG-mediated genetic transformation may prove to be of general utility in performing genetic transformation in a wide variety of organisms.

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