Functions of Conserved Motifs in the RNA-dependent RNA Polymerase of a Yeast Double-stranded RNA Virus

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1998 Apr 29

PMID 9557735

Citations 18

Authors

E Routhier

J A Bruenn

Affiliations

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Abstract

At least eight conserved motifs are visible in the totivirus RNA-dependent RNA polymerase (RDRP). We have systematically altered each of these in the Saccharomyces cerevisiae double-stranded RNA virus ScVL1 by substituting the conserved motifs from a giardiavirus. The results help define the conserved regions of the RDRP involved in polymerase function and those essential for other reasons.

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